Anubhav Pratap-Singh

Assistant Professor

Research Interests

Agri-food Transformation Products
cold plasma
food engineering
food processing
Functional Foods
heat transfer
high pressure
mass transfer
novel non-thermal processing
Nutriceuticals and Functional Foods
pulsed light

Relevant Degree Programs


Research Methodology

pulsed light
cold plasma


Master's students
Doctoral students
Postdoctoral Fellows
I support public scholarship, e.g. through the Public Scholars Initiative, and am available to supervise students and Postdocs interested in collaborating with external partners as part of their research.
I am open to hosting Visiting International Research Students (non-degree, up to 12 months).
I am interested in hiring Co-op students for research placements.

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Graduate Student Supervision

Master's Student Supervision (2010 - 2021)
Double (iron and zinc) fortified black tea: assessing the bioaccessibility and bioavailability using spray drying microencapsulation technology (2021)

Fortification of black tea with iron and zinc has the potential to reduce the prevalence of iron and zinc deficiency in the developing world. Tea is an ideal vehicle for food fortification because it is the second most consumed beverage globally, aside from water, and is consumed throughout the world independent of socioeconomic strata. Unfortunately, polyphenolic compounds present in tea form complexes with iron, which cause colour changes. The formation of this intensely blue-purple non-bioavailable iron-polyphenol complex is a barrier to consumer acceptance and the public health effectiveness of iron-fortified tea.The objective of this study was to develop and assess the fortification of tea with microencapsulated iron and zinc to increase their absorption, and that could prevent the formation of the iron-polyphenol complex in tea. Whey protein isolate and Eudraguard® that either provide gastric and intestinal protection and increase bioaccessibility of iron and zinc in the human body, were used as coating materials for the development of the microencapsulated iron and zinc. A response surface design was used to optimize the encapsulation efficiency of iron and zinc in the microcapsules. The microcapsules were subjected through a simulated gastric and intestinal digestion, whereas the microcapsules showed higher resistance to intestinal conditions.Absorption studies performed using a Caco-2 cell model revealed that the iron delivered through the microcapsules increased cellular absorption by 73%. Zinc from the microcapsules also increased cellular absorption by 81%. The iron-polyphenol complex is dependent on the pH, therefore, the use of MES and PIPES buffers was investigated for the measurement of the iron-polyphenol complex formation. The results show that MES buffer at 0.2M and pH 5.5 can be used to quantify the iron-polyphenol complex in a polyphenolic model system (gallic acid), closely resembling tea. The prevention of the iron-polyphenol complex formation was further investigated with the microencapsulated iron and zinc using a gallic acid and brewed tea. The microcapsules slowed down the formation of iron-polyphenol complex in tea by 60% within 30 minutes of tea brewing. The results of this thesis have the potential to guide the path to reduce micronutrient deficiencies, through fortification of commonly consumed tea with iron and zinc.

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Effect of vacuum microwave dehydration on the off-flavour intensity and functionality of pea proteins (2020)

Pea (Pisum sativum L.) has garnered recent attention as a plant-protein source due to its high protein content, nutrient density and low allergenicity. However, pea proteins are difficult to incorporate into food formulations due to undesirable green, grassy and beany aromas and limited functional properties. Direct steam injection is often used in the food industry to decrease off-flavour intensity of pea proteins and improve functional properties. However, induced cooked off-flavours and nutrient losses that are attributed to heating warrant exploration of an alternative process. This research examined the applicability of vacuum microwave dehydration as a pre-processing step for plant protein for use during non-dairy alternative production. In this thesis, effects of the following process parameters: initial moisture content (5-425% dry basis), vacuum level (40-200 Torr), specific power (10-200 W/g), and process time (1-50 minutes) on volatile compound concentration, functionality and quality parameters such as available lysine and colour, were analyzed. Increasing initial moisture content decreased (p
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Reducing off-flavour in plant protein isolates by lactic acid fermentation (2020)

Pea is a nutritious legume that can be used as an animal protein substitute. However, the unpleasant greeny aroma severely inhibits the application of pea-derived proteins as a food ingredient. This study aims at improving the aroma of pea protein isolates (PPIs) with an aim to employ these PPIs for plant-based dairy substitute production. Major objective of this study is to establish the applicability of lactic acid fermentation (LAF) in eliminating unpleasant aroma from PPIs while maintaining the functional properties and protein quality. We hypothesize that LAF treatment can reduce the undesirable aroma of pea protein isolates by virtue of desirable bacterial action on plant proteins. A solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC-MS) method was developed in this study to identify and quantify the volatile compound profile of plant-based protein. Total concentration of volatile compounds belonging to aldehyde, ketone and alcohol group in the pea, soy and brown rice protein is analyzed and compared. Different LAF treatments are performed with Lactobacillus plantarum, Lactobacillus casei and mixed strains of probiotics. The protein quality of treated and untreated protein sample is analyzed by the Bradford protein assay and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Evolution of functional properties including emulsifying properties, foaming properties, water holding and oil binding capacities of samples with different times of lactic acid fermentation treatments are also analyzed. Based on the experimental results, the water-soluble protein content decreased with the increase of fermentation time, with protein configuration majorly altered beyond 15-hour of fermentation. Thus, a 10-hour L. plantarum fermentation was found optimal in eliminating off-flavour while maintaining protein quality and functional properties. For the optimal treatment, around 42% aldehyde and 64% ketone content were removed, and a small amount of alcohol was produced. This change of the aroma compound profile is considered desirable for dairy substitute production. Results from a descriptive analysis sensory test showed that LAF treatment successfully decreased the overall aroma and flavour intensity in PPIs. The results of this study could be used by the Canadian Food Industry for pre-treating pea protein isolates, before using them as an ingredient in plant-based dairy substitutes.

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