Carolyn Janet Brown
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Affiliations to Research Centres, Institutes & Clusters
X-chromosome inactivation occurs early during mammalian development to transcriptionally silence one of the pair of X chromosomes in females, thereby achieving dosage equivalence with males who have a single X chromosome and the sex-determining Y chromosome. Research in the Brown lab is directed towards understanding both the mechanisms involved in the inactivation process and the clinical implications of X-chromosome inactivation in females.
X-chromosome inactivation is a truly remarkable example of both epigenetic determination (one of a pair of essentially identical chromosomes is chosen to be silenced) and of cell memory (the choice of chromosome inactivated is stably inherited through subsequent somatic divisions for the life of the individual). Our research focusses on two aspects of inactivation: (1) the establishment of inactivation by a long non-coding RNA (lncRNA); and (2) how genes evade the silencing process.
(1) The XIST gene is the only gene that is expressed from the inactive but not from the active X chromosome. This unique gene encodes a 17 kb alternatively spliced, processed transcript which is not translated into a protein but which remains in the nucleus where it associates with the inactive X chromosome. XIST was one of the first lncRNAs to be discovered, and understanding how it functions can reveal parallels for the growing number of lncRNAs being described. We have established a model of XIST function using an inducible XIST transgene in human somatic cells, which allows us to determine how XIST is able to recruit the assembly of silent chromatin on the chromosome from which it is expressed.
(2) Surprisingly, over 20% of human X-linked genes continue to show significant expression from the inactive X chromosome. Therefore, these genes continue to have dosage differences between males and females and are likely to contribute to the phenotype of X-chromosome aneuploidies and some portion of sexually dimorphic traits. In addition to comparison of male and female cells and published datasets, we utilize somatic cell hybrids that allow us to distinguish expression from the active and inactive X chromosome. In collaboration with the Simpson and Wasserman groups at the Centre for Molecular Medicine and Therapeutics at the B.C. Children’s Hospital we are incorporating human DNA into mouse cells to identify the DNA elements involved in spreading (or blocking) silencing along the chromosome. We are also collaborating with Dr. Wendy Robinson’s research group at the B.C. Children’s Hospital to examine the impact of the second X chromosome in female extra-embryonic tissues.
Overall, our goal is to understand the interplay between DNA, RNA and chromatin that underlies silencing of a chromosome and use this knowledge to understand epigenetic gene regulation and the gene expression differences between males and females.
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Graduate Student Supervision
Doctoral Student Supervision
Dissertations completed in 2010 or later are listed below. Please note that there is a 6-12 month delay to add the latest dissertations.
X-chromosome inactivation (XCI) is the process by which one of the X chromosomes in XX females is silenced to express similar levels of X-linked genes with XY males. This silencing is incomplete as some genes escape from XCI and other genes vary their XCI status across populations, tissues or samples. Here I derive consensus XCI status calls in humans, extend XCI status calls across species, and determine the relationship between XCI status and various epigenetic marks.I aggregated XCI status calls from multiple studies, deriving XCI status calls for 639 human genes. I found 12% of genes escaping from XCI, 8% variably escaping XCI, and 7% discordant across studies. To make XCI status calls across species I obtained DNA methylation data for 12 species, allowing us to generate an average of 387 XCI calls per species. Overall, 12% of genes escaped XCI, with mouse an outlier with only 5%. Of the genes with predictions across at least four species, 74.8% of them were entirely consistent and only 6% had more than one inconsistent species. Many genes were seen to have primate-specific escape from XCI, while only one gene had an artiodactyla-specific XCI status. The consensus XCI status calls were compared to DNA methylation and commonly analyzed histone marks. I found the expected trend where repressive marks were enriched at genes subject to XCI and activating marks were enriched at genes escaping XCI; however, the histone marks had a large overlap between levels seen at genes subject to XCI and genes escaping from XCI. Only DNAme could accurately predict an individual gene’s XCI status. I combined the marks and found that we could make XCI status calls with 75% accuracy for genes escaping from XCI and 90% accuracy for genes subject to XCI. The marks with the greatest contribution to this predictor were DNAme, H3K27me3 and H3K4me3. The results of these projects further our understanding of which genes escape from XCI, which may be important for analysis of sexual dimorphism and further provide us a means to examine how silencing may be regulated in humans and across mammals.
X-chromosome inactivation establishes dosage compensation between the sexes of eutherian mammals through the long non-coding RNA gene, XIST. During development one of the female X chromosomes up-regulates XIST, which coats that chromosome and causes the large-scale silencing of genes. The X chromosome being inactivated by XIST is repositioned within the nucleus, condensed and enriched with heterochromatin associated factors. Understanding the mechanisms of how XIST functions has provided insights into how non-coding RNAs regulate cellular biology, the process of X-chromosome inactivation and novel tools for regulating the epigenome.The function of XIST was investigated using an inducible XIST cDNA construct integrated into the autosome of a male fibrosarcoma cell line, HT1080. The chromatin domain surrounding the XIST loci had a significant effect on its activity, and of all the autosomal loci the 8p integration site functioned most effectively and was thus used for further studies. A series of isogenic inducible partial XIST constructs were created by modifying the Full length XIST construct in 8p to study the importance of individual regions of XIST. The functions dependent on each region of XIST were identified and the relationships between these identified processes were then examined through the use of chemical inhibitors. XIST silencing of genes was demonstrated to depend upon two distinct regions at the extreme ends of the transcript, but the internal sequences spanning these regions were dispensable. Silencing occurred without obvious dependence on chromatin modifications such as those established by the two polycomb group complexes, that in turn relied on ¬distinct regions of XIST suggesting entirely independent mechanisms. Both polycomb complexes were crucial, along with additional elements, for the recruitment of additional heterochromatin factors. This study in human differentiated cells yielded important insights beyond those seen in mouse differentiating cells. The results of this thesis revealed the regions of XIST that were both crucial and dispensable for its activity, and offer novel insights into the mechanisms that lead to chromosome inactivation.
The process of X-chromosome inactivation achieves dosage compensation betweenmammalian males and females. In females one X chromosome is transcriptionally silencedthrough a variety of epigenetic modifications including DNA methylation. Most X-linked genesare subject to X-chromosome inactivation and only expressed from the active X chromosome.On the inactive X chromosome, the CpG island promoters of genes subject to X-chromosomeinactivation are methylated in their promoter regions, while genes which escape from Xchromosomeinactivation have unmethylated CpG island promoters on both the active andinactive X chromosomes.The first objective of this thesis was to determine if the DNA methylation of CpG islandpromoters could be used to accurately predict X chromosome inactivation status. The secondobjective was to use DNA methylation to predict X-chromosome inactivation status in a varietyof tissues. A comparison of blood, muscle, kidney and neural tissues revealed tissue-specificX-chromosome inactivation, in which 12% of genes escaped from X-chromosome inactivation insome, but not all, tissues. X-linked DNA methylation analysis of placental tissues predicted fourtimes higher escape from X-chromosome inactivation than in any other tissue. Despite thehypomethylation of repetitive elements on both the X chromosome and the autosomes, nochanges were detected in the frequency or intensity of placental Cot-1 holes.The third objective of this thesis was to use DNA methylation to investigate X-chromosomeinactivation in female samples with chromosomally abnormal karyotypes. The spread of Xchromosomeinactivation into the autosomal portion of six unbalanced X;autosometranslocations revealed similarities between X;autosome translocations involving the sameautosome and therefore suggested a role for DNA sequence in influencing X-chromosomeinactivation status of genes. Autosomal genes that escaped from inactivation were found tohave significantly lower L1 and LTR but higher Alu content than genes which were subject toinactivation. Lastly, DNA methylation was used to predict the number of inactive Xchromosomes in triploid placental samples. Triploid samples provide an excellent system inwhich to study the counting step of X-chromosome inactivation and DNA methylation analysisprovides a means to determine the number of inactive X chromosomes using only a DNAsample.
X-chromosome inactivation ensures equal expression of mammalian male and female X-linked genes by transcriptionally silencing one X chromosome in each female cell. The pivotal molecule responsible for the silencing is a long non-coding RNA XIST; however, an all-encompassing model explaining how XIST induces silencing of the whole X chromosome is yet to emerge. This thesis aims to broaden our understanding of XIST action in humans by leveraging an inducible XIST transgene capable of silencing downstream reporters to identify sequences within XIST and XIST-interacting proteins critical for gene silencing.First, we demonstrate that the repeat A region of XIST is necessary and sufficient to induce gene silencing, at least locally, irrespective of the makeup of the surrounding chromatin, and that XIST induces silencing of a distal gene in one of the HT1080 cell lines. Second, we show that individual repeats of a consensus repeat A sequence contribute additively to silencing. Mutations within a construct consisting of two repeat A units both demonstrate that the two palindromic sequences within the repeat A units spanning ‘ATCG’ and ‘ATAC’ tetranucleotides are critical for repeat A function and add to the evidence that the first palindrome forms a hairpin, rather than engaging in pairing between repeat A units.Third, we explore which proteins are critical for XIST-induced silencing. We show that histone deacetylation, an early mark of an X-chromosome inactivation, is likely a consequence, and not the cause of XIST-induced silencing. We next demonstrate that in the transgenic HT1080 system, gene silencing is not accompanied by recruitment of the H3K27me3 repressive histone mark and XIST induces silencing independently of its previously reported associations with the polycomb repressive complex 2 (PRC2). Finally, we performed siRNA-mediated knock-down of 31 proteins previously implicated to play a role in X-chromosome inactivation. Our results show that proteins involved in XIST RNA localization (YY1), chromatin organization (SATB2, HNRNPU), and cell cycle (ATM), as well as an E3 ubiquitin ligase (SPOP) contribute to XIST-induced gene silencing in the HT1080 system. Thus, we demonstrate that the repeat A alone induces gene silencing and identify candidate pathways critical for its function.
Mammalian dosage compensation of X-linked genes is achieved between XX females and XY males by silencing one of the two X chromosomes. It is the expression of a functional non-coding RNA transcript, XIST that is responsible for the initiation of silencing during X-chromosome inactivation. XIST expression and subsequent in cis localization to the future inactive X chromosome initiates a cascade of epigenetic events that leads to the formation of facultative heterochromatin. The exact role the XIST RNA plays in establishing and maintaining the inactive state is uncertain. It is hypothesized that the XIST RNA sets up a repressive nuclear compartment and transcriptionally silences through the recruitment of factors required for setting up the heterochromatic state of the inactive X. In this thesis I address XIST/Xist’s recruitment of factors with two separate approaches: 1) I ask whether Xist, in the absence of silencing is able to recruit epigenetic marks in a somatic cell hybrid system, 2) I evaluate a system whereby the XIST RNA is tagged and can be isolated to identify novel RNA-protein interactions. To assess epigenetic features that may be directly recruited by the expression of the XIST/Xist RNA, I have analyzed mouse/human somatic cell hybrids where XIST/Xist expression and silencing are disconnected. Loss of active chromatin marks, H3 acetylation and H3 lysine 4 methylation, was not observed with XIST/Xist expression; nor was there a gain of DNA methylation or the silencing of the Cot-1 fraction. Therefore, these marks of heterochromatin are not solely dependent upon XIST/Xist expression in a somatic cell.The isolation of the XIST RNA with its interacting partners would allow us to better understand the mechanism by which XIST acts to silence the inactive X. I have developed a MS2 stem loop-tagged XIST RNA and integrated it into an inducible XIST system. The MS2 tagging system is a valid system for pulling out the XIST RNA and identifying interacting proteins since the tagged RNA was able to interact with whatever factor(s) are required to silence the Cot-1 fraction and form a localized RNA signal.
Dosage compensation in female mammals is achieved by XIST/Xist RNA mediatedtranscriptional silencing of one of the two X chromosomes. Several developmental specific cis-acting regulatory elements for mouse XIST have been demonstrated. One of these regulatory elements is the mouse Tsix locus, which is transcribed antisense to Xist and represses Xist on one ofthe X chromosomes at the onset of X inactivation. A transcript antisense to human XIST has been shown; however, its functional significance has been repeatedly challenged. My thesis aims to uncover cis-acting regulatory elements for human XIST and determine whether the elements arecomparable to those found in mice.Currently, multi-copy integrations of a transgene containing human XIST into male mouse embryonic stem (ES) cells or into male human fibrosarcoma cells are the model systems of choice for studying the initiation of human X inactivation because the transcription antisense to humanXIST can be detected by RT-PCR from the transgenes. Using DNase I hypersensitivity (HS) mapping, I found one HS site on the human transgene in mouse ES cells located approximately 11 kb downstream of XIST3’ end. While this HS site does not correspond to the transcription starts of TSIX previously described, it encompasses a small cluster of CTCF binding sites based on in silico search. Besides the downstream HS site, I discovered two HS sites in differentiated cell lines. One of the HS sites is immediately upstream of the XlST transcription start site. The other HS site (HS 101), located approximately 65 kb upstream of human XIST transcription start, resides within a region that shares above 70% sequence identity with cow and dog but not mouse. I analyzed these upstream HS sites and found that HS 101 exhibits bi-directional promoter and enhancer activity. My thesis revealed three previously unidentified HS sites flanking the human XIST locus; the presence of only one ES cell specific HS site downstream of XIST3’ end is in sharp contrast to theseven sites reported in mouse. The results suggest that mouse Xist and human XIST are regulated differently. To account for the differences in regulatory elements, I propose an alternative model for human XIST regulation.
Master's Student Supervision
Theses completed in 2010 or later are listed below. Please note that there is a 6-12 month delay to add the latest theses.
X inactivation is the process of silencing one of the two X chromosomes in mammalian female cells in order to equalise the dosage of X-linked genes with males. The process is initiated by the long noncoding RNA XIST, which is transcribed from the future inactive X and localises to it in cis. How XIST RNA is able to localise to the X chromosome is not well defined. The aim of the current study was to deduce mechanisms of XIST RNA localisation. This was addressed in various ways, including 1) testing the ability of an XIST transgene integrated into a variety of autosomes to localise to those autosomes, as opposed to the X chromosome; 2) assessing the ability of XIST transgenes with different regions deleted to localise, in order to identify sequences required for localisation; and 3) knocking down various proteins implicated in X inactivation in order to assess any effect on the ability of XIST to localise. We find that the XIST transgene is able to localise to a wide variety of different autosomes and furthermore, is able to direct the enrichment of the histone variant macroH2A on an autosome and the deposition of a repressive histone modification, H3K27me3, onto an autosome. We also find that a region of XIST encompassing repeats B and C, and sequences downstream of exon 1 are involved in localising XIST RNA, and that they do so in a redundant fashion. Lastly, we show that the knockdown of five proteins - YY1, hnRNP-U, SPOP, CUL3 and ASH2L - prevent the formation of an intact XIST focus. The results presented here add to the limited knowledge of how XIST RNA is able to localise, an essential step in the process of X chromosome inactivation.
X-chromosome inactivation is a mechanism that has evolved in mammalian femalesallowing dosage compensation of X-linked genes. A region of the X chromosome called the Xinactivationcentre (XIC) is required for X inactivation to occur. Within this region is a long noncodingRNA, XIST/Xist, which is upregulated on the future inactive X and initiates silencing. Amajor questions in the field of X inactivation is how XIST/Xist is regulated, becoming expressedon the inactive X and silenced on the active X. Much of what we currently know about XIST/Xistregulation comes from studies using mice, however, differences in conservation of the XIC andexpression patterns of the major mouse Xist regulator, Tsix, indicate that humans and mice mayregulate XIST/Xist differently. The objective of this thesis was to identify regulatory elementsthat are important for regulation of XIST in humans.Since regulatory elements controlling XIST are believed to reside within the XIC, wesearched the XIC and identified two inactive X specific regulatory elements within the 5’ end ofXIST using DNase I hypersensitivity mapping. We found one of the hypersensitive sites to beacting as an alternative P2 promoter for XIST which contains an upstream antisense promoter,P2as. The second hypersensitive site was associated with alternative splicing and inclusion oftwo novel exons for XIST. Interestingly, both P2 and the novel alternative splicing result intranscripts that lack functional domains of XIST. An additional candidate regulator is the region3’ of XIST due to the importance of Tsix in mice. We found that transcription 3’ of XIST insomatic cells is low level sense transcription so we believe this to be leaky XIST rather thanTSIX. In human embryonic stem cells we found an antisense transcript that extends the fulllength of XIST providing the first evidence for mouse-like TSIX in humans but very low-levels ofthis transcript argue against regulatory ability. Taken together, our results highlight thedifferences between mouse and human X inactivation and indicate that XIST transcription ismore complex than previously thought, generating XIST molecules that lack functional domains.
X-chromosome inactivation is the process by which mammalian females achieve dosage compensation with males by silencing one of the two X chromosomes in female cells. Despite the chromosome-wide inactivation, a significant proportion of genes on the X chromosome in humans remain expressed on the inactive X chromosome. It has been long hypothesized that the genomic context plays an important role in influencing whether a gene is subject to or escapes from X-chromosome inactivation; however, cis-regulatory elements involved in X-chromosome inactivation have not yet been identified. The objective of this thesis was to identify DNA elements that promote the escape of genes from X-chromosome inactivation in the human genome, through analyzing the X-chromosome inactivation statuses of human transgenes integrated at the Hprt locus on the mouse X chromosome and identifying the transgenes that escape from X-chromosome inactivation. DNA methylation was used to assess the inactivation status of 74 human reporter constructs comprising over 1.5 Mb of DNA. Transgenes that show low promoter DNA methylation in males and females would be potential escape genes. Of the 47 genes examined, only the PHB gene showed female DNA hypomethylation approaching the level seen in males, and escape from X-chromosome inactivation was verified by the demonstration of expression from the inactive X chromosome in females with non-random X-chromosome inactivation. Analysis on the repeat element content of five BAC-derived transgenes subject to X-chromosome inactivation suggested that local LINE-1 and Alu densities were insufficient to determine whether a gene would be subject to X-inactivation. Interestingly, CpG islands not associated with promoters also showed female-specific DNA hypermethylation, suggesting a dominant effect of X-chromosome inactivation on the regulation of DNA methylation. Different human transgenes show a differential capacity to accumulate DNA methylation when integrated into the identical location on the inactive X chromosome, indicating the presence of additional cis-acting epigenetic modulators. As only one of the human transgenes analyzed escaped from X-chromosome inactivation, we conclude that elements involved in ongoing expression from the inactive X are rare in the human genome and that mouse X-chromosome inactivation is very effective in silencing human transgenes.
- Multiple distinct domains of human XIST are required to coordinate gene silencing and subsequent heterochromatin formation (2022)
Epigenetics & Chromatin,
- A cross-cohort analysis of autosomal DNA methylation sex differences in the term placenta (2021)
- Contribution of epigenetic changes to escape from X-chromosome inactivation (2021)
- Contribution of genetic and epigenetic changes to escape from X-chromosome inactivation (2021)
Epigenetics & Chromatin,
- Cross-species examination of X-chromosome inactivation highlights domains of escape from silencing (2021)
Epigenetics & Chromatin, 14 (1)
- Independent domains for recruitment of PRC1 and PRC2 by human XIST (2021)
PLOS Genetics, 17 (3), e1009123
- Genes that escape from X‐chromosome inactivation: Potential contributors to Klinefelter syndrome (2020)
American Journal of Medical Genetics Part C: Seminars in Medical Genetics,
- Beyond sequence homology: Cellular biology limits the potential of XIST to act as a miRNA sponge (2019)
- How do genes that escape from X‐chromosome inactivation contribute to Turner syndrome? (2019)
American Journal of Medical Genetics Part C: Seminars in Medical Genetics, 181 (1), 28--35
- Human cis-acting elements regulating escape from X-chromosome inactivation function in mouse (2018)
Human Molecular Genetics, 27 (7), 1252--1262
- The eXceptional nature of the X chromosome (2018)
Human Molecular Genetics,
- Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST (2015)
Genome Biology, 16 (1)
- Landscape of DNA methylation on the X chromosome reflects CpG density, functional chromatin state and X-chromosome inactivation (2015)
Human Molecular Genetics, 24 (6), 1528-1539
- Unique somatic and malignant expression patterns implicate PIWI-interacting RNAs in cancer-type specific biology (2015)
Scientific Reports, 5
- Variable escape from X-chromosome inactivation: Identifying factors that tip the scales towards expression (2014)
BioEssays, 36 (8), 746--756
- Additional annotation enhances potential for biologically-relevant analysis of the Illumina Infinium HumanMethylation450 BeadChip array (2013)
Epigenetics and Chromatin, 6 (1)
- Analysis of expressed SNPs identifies variable extents of expression from the human inactive X chromosome (2013)
Genome Biol, 14 (11), R122
- DNA Methylation is Globally Disrupted and Associated with Expression Changes in COPD Small Airways (2013)
Am J Respir Cell Mol Biol,
- EYA4 is inactivated biallelically at a high frequency in sporadic lung cancer and is associated with familial lung cancer risk (2013)
- Spread of X-chromosome inactivation into autosomal sequences: role for DNA elements, chromatin features and chromosomal domains (2013)
Hum Mol Genet,
- Translating dosage compensation to trisomy 21 (2013)
Nature, 500 (7462), 296-300
- XIST-induced silencing of flanking genes is achieved by additive action of repeat a monomers in human somatic cells (2013)
Epigenetics & Chromatin, 6 (1), 23
- Patterns of placental development evaluated by X chromosome inactivation profiling provide a basis to evaluate the origin of epigenetic variation (2012)
Human Reproduction, 27 (6), 1745-1753
- Targeting of >1.5 Mb of human DNA into the mouse X chromosome reveals presence of cis-acting regulators of epigenetic silencing (2012)
Genetics, 192 (4), 1281-1293
- Chromosome-wide DNA methylation analysis predicts human tissue-specific X inactivation (2011)
Human Genetics, 130 (2), 187-201
- Epigenetic Impacts on Neurodevelopment: Pathophysiological Mechanisms and Genetic Modes of Action (2011)
Pediatric Research, 69 (5 Par), 92R-100R
- Human cancer long non-coding RNA transcriptomes (2011)
PLoS ONE, 6 (10)
- S100B and neurofibromin immunostaining and X-inactivation patterns of laser-microdissected cells indicate a multicellular origin of some NF1-associated neurofibromas (2011)
Journal of Neuroscience Research, 89 (9), 1451-1460
- The functional role of long non-coding RNA in human carcinomas (2011)
Molecular Cancer, 10
- X-chromosome inactivation: Molecular mechanisms from the human perspective (2011)
Human Genetics, 130 (2), 175-185
- Acquired TNFRSF14 mutations in follicular lymphoma are associated with worse prognosis (2010)
Cancer Research, 70 (22), 9166-9174
- Active chromatin marks are retained on X chromosomes lacking gene or repeat silencing despite XIST/Xist expression in somatic cell hybrids (2010)
PLoS ONE, 5 (5)
- Identification of regulatory elements flanking human XIST reveals species differences (2010)
BMC Molecular Biology, 11
- Erratum: X chromosome inactivation: Heterogeneity of heterochromatin (Biochem. Cell. Biol (2008) 86 (370-379)) (2009)
Biochemistry and Cell Biology, 87 (1)
- Getting to the center of X-chromosome inactivation: The role of transgenes (2009)
Biochemistry and Cell Biology, 87 (5), 759-766
- Inactive X chromosome-specific reduction in placental DNA methylation (2009)
Human Molecular Genetics, 18 (19), 3544-3552
- Methylated DNA Immunoprecipitation (2009)
Journal of Visualized Experiments, (23)
- A skewed view of X chromosome inactivation (2008)
Journal of Clinical Investigation, 118 (1), 20-23
- Correction: BCoR-L1 variation and breast cancer (2008)
Breast Cancer Research, 10 (5)
- Epigenetics of cancer progression (2008)
Pharmacogenomics, 9 (2), 215-234
- Prognostic significance of secondary cytogenetic alterations in follicular lymphomas (2008)
Genes Chromosomes and Cancer, 47 (12), 1038-1048
- X chromosome inactivation: Heterogeneity of heterochromatin (2008)
Biochemistry and Cell Biology, 86 (5), 370-379
- A comprehensive analysis of common copy-number variations in the human genome (2007)
American Journal of Human Genetics, 80 (1), 91-104
- A cross-species comparison of X-chromosome inactivation in Eutheria (2007)
Genomics, 90 (4), 453-463
- BCoR-L1 variation and breast cancer (2007)
Breast Cancer Research, 9 (4), R54
- Comparative Cancer Epigenomics (2007)
- Inducible XIST-dependent X-chromosome inactivation in human somatic cells is reversible (2007)
Proceedings of the National Academy of Sciences of the United States of America, 104 (24), 10104-10109
- Reply to Dr. Robert A. Hegele  (2007)
American Journal of Human Genetics, 81 (2)
- Skewed X-chromosome inactivation is associated with primary but not secondary ovarian failure (2007)
American Journal of Medical Genetics, Part A, 143 (9), 945-951
- Epigenomics: Mapping the methylome (2006)
Cell Cycle, 5 (2), 155-158
- Mechanisms of X-chromosome inactivation (2006)
Frontiers in Bioscience, 11 (1 P.4), 852-866
- Investigations of the genomic region that contains the clf1 mutation, a causal gene in multifactorial cleft lip and palate in mice (2005)
Birth Defects Research Part A - Clinical and Molecular Teratology, 73 (2), 103-113
- Silencing of the mammalian X chromosome (2005)
Annual Review of Genomics and Human Genetics, 6, 69-92
- A Unique Patient with an Ullrich-Turner Syndrome Variant and Mosaicism for a Tiny r(X) and a Partial Proximal Duplication 1q (2004)
American Journal of Medical Genetics, 124 A (3), 303-306
- The dynamics of X-inactivation skewing as women age (2004)
Clinical Genetics, 66 (4), 327-332
- A stain upon the silence: genes escaping X inactivation (2003)
Trends in Genetics, 19 (8), 432-438
- Beyond sense: The role of antisense RNA in controlling Xist expression (2003)
Seminars in Cell and Developmental Biology, 14 (6), 341-347
- Characterization of expression at the human XIST locus in somatic, embryonal carcinoma, and transgenic cell lines (2003)
Genomics, 82 (3), 309-322
- Forming facultative heterochromatin: Silencing of an X chromosome in mammalian females (2003)
Cellular and Molecular Life Sciences, 60 (12), 2586-2603
- Methylation of ZNF261 as an assay for determining X chromosome inactivation patterns  (2003)
American Journal of Medical Genetics, 120 A (3), 439-441
- Skewed X-chromosome inactivation is associated with trisomy in women ascertained on the basis of recurrent spontaneous abortion or chromosomally abnormal pregnancies (2003)
American Journal of Human Genetics, 72 (2), 399-407
- X-chromosome inactivation (XCI) patterns in placental tissues of a paternally derived bal t(X;20) case (2003)
American Journal of Medical Genetics, 118 A (1), 29-34
- Ectopic XIST transcripts in human somatic cells show variable expression and localization (2002)
Cytogenetic and Genome Research, 99 (1-4), 92-98
- Lack of expression of XIST from a small ring X chromosome containing the XIST locus in a girl with short stature, facial dysmorphism and developmental delay (2002)
European Journal of Human Genetics, 10 (1), 44-51
- Relationship of XIST expression and responses of ovarian cancer to chemotherapy. (2002)
Molecular cancer therapeutics, 1 (10), 769-776
- Variability of X chromosome inactivation: effect on levels of TIMP1 RNA and role of DNA methylation (2002)
Human Genetics, 110 (3), 271-278
- Biology of the X chromosome: Molecular and clinical correlates (2001)
Seminars in Reproductive Medicine, 19 (2), 123-124
- Equality of the sexes: Mammalian dosage compensation (2001)
Seminars in Reproductive Medicine, 19 (2), 125-132
- Skewed X inactivation and recurrent spontaneous abortion (2001)
Seminars in Reproductive Medicine, 19 (2), 175-181
- Unravelling the complex genetics of cleft lip in the mouse model (2001)
Mammalian Genome, 12 (6), 426-435
- X chromosome-specific cDNA arrays: Identification of genes that escape from X-inactivation and other applications (2001)
Human Molecular Genetics, 10 (1), 77-83
- An association between skewed X-chromosome inactivation and abnormal outcome in mosaic trisomy 16 confined predominantly to the placenta (2000)
Clinical Genetics, 58 (6), 436-446
- Determination of X-Chromosome Inactivation Status Using X-Linked Expressed Polymorphisms Identified by Database Searching (2000)
Genomics, 65 (1), 9-15
- Involvement of the X chromosome in non-Hodgkin lymphoma (2000)
Genes Chromosomes and Cancer, 28 (3), 246-257
- The causes and consequences of random and non-random X chromosome inactivation in humans (2000)
Clinical Genetics, 58 (5), 353-363
- Extremely skewed X-chromosome inactivation is increased in women with recurrent spontaneous abortion  (1999)
American Journal of Human Genetics, 65 (3), 913-917
- Polymorphic X-chromosome inactivation of the human TIMP1 gene (1999)
American Journal of Human Genetics, 65 (3), 699-708
- Skewed X-chromosome inactivation: Cause or consequence (1999)
Journal of the National Cancer Institute, 91 (4), 304-305
- Induction of XIST expression from the human active X chromosome in mouse/human somatic cell hybrids by DNA demethylation (1998)
Nucleic Acids Research, 26 (12), 2935-2940
- Stabilization and localization of Xist RNA are controlled by separate mechanisms and are not sufficient for X inactivation (1998)
Journal of Cell Biology, 142 (1), 13-23
- Expression of genes from the human active and inactive X chromosomes (1997)
American Journal of Human Genetics, 60 (6), 1333-1343
- Skewed X-chromosome inactivation is common in fetuses or newborns associated with confined placental mosaicism (1997)
American Journal of Human Genetics, 61 (6), 1353-1361
- XIST expression and X-chromosome inactivation in human preimplantation embryos (1997)
American Journal of Human Genetics, 61 (1), 5-8
- Evidence that heteronuclear proteins interact with the XIST RNA in vitro (1996)
Somatic Cell and Molecular Genetics, 22 (5), 403-417
- Role of the X chromosome in cancer. (1996)
Journal of the National Cancer Institute, 88 (8), 480-482
- Direct detection of non-random X chromosome inactivation by use of a transcribed polymorphism in the XIST gene (1995)
European Journal of Human Genetics, 3 (6), 333-343
- Identification of a PIG-A related processed gene on chromosome 12 (1995)
Human Genetics, 95 (6), 691-697
- The DXS423E gene in Xp11.21 escapes X chromosome inactivation (1995)
Human Molecular Genetics, 4 (2), 251-255
- Small marker X chromosomes lack the X inactivation center: Implications for karyotype/phenotype correlations (1994)
American Journal of Human Genetics, 55 (1), 87-95
- The human X-inactivation centre is not required for maintenance of X-chromosome inactivation (1994)
Nature, 368 (6467), 154-156
- 2.6 Mb YAC contig of the human X inactivation center region in Xq13: Physical linkage of the RPS4X, PHKA1, XIST and DXS128E genes (1993)
Human Molecular Genetics, 2 (8), 1105-1115
- Characterization of a small supernumerary ring X chromosome by fluorescence in situ hybridization (1993)
American Journal of Medical Genetics, 47 (8), 1153-1156
- Epigenetic and chromosomal control of gene expression: Molecular and genetic analysis of X chromosome inactivation (1993)
Cold Spring Harbor Symposia on Quantitative Biology, 58, 315-322
- Evolutionary conservation of possible functional domains of the human and murine XIST genes (1993)
Human Molecular Genetics, 2 (6), 663-672
- Mapping of the distal boundary of the X-inactivation center in a rearranged X chromosome from a female expressing XIST (1993)
Human Molecular Genetics, 2 (7), 883-887
- Molecular and Genetic Studies of Human X Chromosome Inactivation (1993)
Advances in Developmental Biology (1992), 2 (C), 37-72
- The interleukin-2 receptor γ chain maps to Xq13.1 and is mutated in X-linked severe combined immunodeficiency, SCIDX1 (1993)
Human Molecular Genetics, 2 (8), 1099-1104
- The human XIST gene: Analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus (1992)
Cell, 71 (3), 527-542
- A gene deleted in Kallmann's syndrome shares homology with neural cell adhesion and axonal path-finding molecules (1991)
Nature, 353 (6344), 529-536
- A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome (1991)
Nature, 349 (6304), 38-44
- Localization of the X inactivation centre on the human X chromosome in Xq13 (1991)
Nature, 349 (6304), 82-84
- Physical mapping of 60DNA markers in the p21.1 → q21.3 region of the human X chromosome (1991)
Genomics, 11 (2), 352-363
- The 56/58 kDa androgen-binding protein in male genital skin fibroblasts with a deleted androgen receptor gene (1991)
Molecular and Cellular Endocrinology, 75 (1), 37-37
- Localization of a gene that escapes inactivation to the X chromosome proximal short arm: Implications for X inactivation (1990)
American Journal of Human Genetics, 46 (2), 273-279
- X chromosome inactivation of the human TIMP gene (1990)
Nucleic Acids Research, 18 (14), 4191-4195
- Androgen receptor locus on the human X chromosome: Regional localization to Xq11-12 and description of a DNA polymorphism (1989)
American Journal of Human Genetics, 44 (2), 264-269
- Gene on short arm of human X chromosome complements murine tsA1S9 DNA synthesis mutation (1989)
Somatic Cell and Molecular Genetics, 15 (2), 173-178
- Noninactivation of a selectable human X-linked gene that complements a murine temperature-sensitive cell cycle defect (1989)
American Journal of Human Genetics, 45 (4), 592-598
- Regional localization of CCG1 gene which complements hamster cell cycle mutation BN462 to Xq11-Xq13 (1989)
Somatic Cell and Molecular Genetics, 15 (1), 93-96
- Genetic mapping of four DNA markers (DXS16, DXS43, DXS85, and DXS143) from the p22 region of the human X chromosome (1988)
Human Genetics, 80 (3), 296-298
- MspI RFLP detected with chromosome-walk clone pXUT23-SE3.2L from DXS16 in Xp22.1-22.3 (1987)
Nucleic Acids Research, 15 (22)