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genetic causes of autism and intellectual disability
Graduate Student Supervision
Doctoral Student Supervision (Jan 2008 - Mar 2019)
Synapses of the central nervous system are specialized points of cell-cell contact that transmit signals from one neuron to another in an efficient manner. A fundamental property of synapses is that they can be altered in response to activity, a process called “synaptic plasticity.” Synaptic activity can cause lasting increases in synaptic strength (long-term potentiation, or ‘LTP’), and decreases in synapse strength (long-term depression, or ‘LTD’).The cell adhesion molecules ‘cadherins’ and their intracellular binding partners β-catenin and δ-catenin are key mediators of synaptic plasticity. The disruption of the cadherin adhesion complex impairs LTP, and increased cadherin stability at synaptic membranes impairs LTD. The role of cadherins in synaptic plasticity has been well studied in the hippocampus, and cadherins have been shown to influence spatial learning and memory. However, little is known about the importance of cadherins in other brain regions, and in other forms of learning. In the first half of this dissertation, I examine the role of the cadherin adhesion complex in cocaine-mediated plasticity in the ventral tegmental area (VTA) of the mesocorticolimbic dopamine circuit. I demonstrate that cadherins play an important role in activity- and cocaine-mediated plasticity in the VTA. Furthermore I find that increasing cadherin localization at the synaptic membranes of VTA dopamine cells impairs AMPA receptor trafficking, synaptic plasticity, and cocaine-mediated behavioural conditioning.Previous work has also shown that the cadherin adhesion complex protein, δ-catenin, can be modified through the addition of the fatty acid, palmitate, to cysteine residues in a process called palmitoylation. δ-catenin palmitoylation results in increased cadherin-δ-catenin interactions and increases in synapse strength. The palmitoylation of δ-catenin is mediated by the palmitoyl acyltransferase, zDHHC5. Although zDHHC5 has been shown to play an important role in synaptic plasticity, its role in neuronal development has not been examined. In the second half of this dissertation, I examine the role of zDHHC5 in dendrite outgrowth and synapse formation, finding that the palmitoylation function and proper localization of zDHHC5 at the plasma membrane of postsynaptic spines are important for the stability of dendritic spines and the formation of excitatory synapses.
A fundamental property of synapses is their ability to change in response to activity, termed ‘synaptic plasticity’. Synaptic activity can cause long-lasting increases in the strength of synapses (long-term potentiation, or ‘LTP’), as well as decreases in synapse strength (long-term depression or ‘LTD’), both of which are believed to be important for learning and memory. The synaptic adhesion molecules ‘cadherins’ and their intracellular binding partner β-catenin have been identified as key mediators of plasticity at synapses. The cadherin adhesion complex is important for maintaining the strength and stability of synapses, and disruption of cadherin function has been shown to impair long-term potentiation (LTP). However, it remains unclear how increases in cadherin adhesion can affect synaptic function and cognition. This is important in light of studies showing that increases in β-catenin levels and mutations in cadherin adhesion complex proteins are associated with many different neurodegenerative diseases, as well as psychiatric disorders such as drug abuse, raising the possibility that aberrant increases in cadherin adhesion may contribute to cognitive impairments in these disorders. In this dissertation, I examine the effects of increases in cadherin adhesion on different forms of synaptic plasticity in the brain. I demonstrate that increases in β-catenin in the hippocampus can stabilize cadherin at the synaptic membrane and abolish long-term depression (LTD) at synapses, leading to significant impairments in spatial memory flexibility and reversal learning. I also demonstrate a role for cadherin in activity- and drug-induced plasticity in the ventral tegmental area (VTA), a region of the brain important for reward learning which is implicated in addiction, and show that cocaine-mediated conditioned place preference results in redistribution of cadherin and AMPA receptors to excitatory synapses onto dopaminergic neurons in the VTA. Together, these results demonstrate that the β-catenin/cadherin adhesion complex plays an important role in several forms of learning and memory, and that aberrant increases in synaptic adhesion can have a detrimental effect on synaptic plasticity and cognitive function.
No abstract available.
No abstract available.
Cell adhesion molecules (CAMs) have emerged as important players in synapse development; however, the precise roles of these proteins at newly formed contacts remain unknown. In this thesis, I begin by providing an overview of synaptic structure and development, as well as a review of our current understanding of how key CAMs and associated proteins fit into this framework.In the second chapter, I demonstrate that members of the postsynaptically localized neuroligin (NL) family of CAMs, including NL1, NL2 and NL3, can trigger the formation of excitatory and inhibitory presynaptic terminals, and that while NL1 is enriched at excitatory contacts, NL2 localizes primarily to inhibitory sites. Neuroligin-mediated enhancement of inhibitory synapse density is blocked by a fusion protein containing the extracellular domain of the presynaptic neuroligin binding partner, neurexin-1β. Furthermore, overexpression of postsynaptic density-95 (PSD-95), a postsynaptic binding partner of neuroligins, results in a shift of NL2 from inhibitory to excitatory synapses. These findings reveal that multiple neuroligins control the number of inhibitory and excitatory synapses, and that localization of NL2 can be altered by scaffolding proteins.In the third chapter, I examine the mechanisms by which NL2 and NL3 are recruited to inhibitory and excitatory synapses, respectively. To this end, I assessed the roles of PSD-95 and gephyrin, a postsynaptic scaffolding molecule localized exclusively to inhibitory synapses, in localizing NL2 and NL3. Knockdown of gephyrin results in a shift of NL2 from inhibitory to excitatory synaptic contacts, while knockdown of PSD-95 leads to a shift of NL2 and NL3 from excitatory to inhibitory contacts. Deletion of a discrete region within the C-terminus of NL2 reveals that the intracellular tail is required for the normal synaptic clustering of this protein. Together, these data suggest that intracellular mechanisms are involved in the synaptic targeting of different neuroligin family members.Overall, these results demonstrate an important role for neuroligins in the development of glutamatergic and GABAergic synapses, and indicate that postsynaptic scaffolding molecules modulate the targeting of neuroligins to distinct postsynaptic compartments. The final chapter of the thesis provides a general discussion relating these findings to other recent advancements in the field.
Master's Student Supervision (2010-2017)
Autism spectrum disorder (ASD) is a pervasive neurodevelopmental disorder primarily characterized by stereotypic behaviors, deficits in social interaction and difficulties with communication. Extensive epidemiological studies suggest a major role for genetics in the etiology of ASD. To date, 600-1,200 human genes have putatively been linked to ASD, including SEMA5A and PTEN. A large number of these ASD-associated genes play a role in the formation, maintenance, elimination or stabilization of synapses, while others are involved in broader elements of neurodevelopment, such as dendrite arborization, dendritogenesis, and soma size. Consistent with neurological dysfunction in ASD are observations that individuals with ASD often have supernumerary synapses, disrupted excitatory/inhibitory balance, and patterns of hypo- and hyper-connectivity compared to the general population. Despite this, many of the neurological functions of ASD-associated genes or gene disruptions remain poorly elucidated.In this study, we examine the role of Sema5A in activity-mediated synapse elimination, notably hippocampal long-term potentiation (LTP) and long-term depression (LTD). We describe the enhanced trafficking of Sema5A to the surface membrane during LTD and the subsequent Sema5A-dependent elimination of excitatory synapses. Furthermore, we demonstrate that Sema5A selectively mediates excitatory—and not inhibitory—synapse elimination, suggesting a mechanism by which the dysregulation of Sema5A could disrupt excitatory/inhibitory balance. Secondly, we describe the role of PTEN in negatively regulating excitatory synapse density, total dendritic arbor length, and soma size. Moreover, we characterize alterations to the neurological functions of PTEN in mature hippocampal neurons following the introduction of ASD-associated single nucleotide variants (SNVs). We demonstrate that most of the ASD-associated PTEN SNVs tested are broadly loss of function, with two notable exceptions: P38H PTEN exhibits a single altered neurological function, while H123Q PTEN phenocopies wild type human PTEN across all measures, further stressing the importance of biological functionalization. Lastly, we establish a PTEN knockdown assay in which PTEN SNVs could be tested for synaptic, dendrite and somal phenotypes. Combined and integrated, the functionalization of ASD-associated genes and gene variants could permit greater accuracy in ASD diagnoses and prognoses, as well as the improved targeting of therapeutic interventions.
The semaphorins are a large protein family, highly conserved across diverse phyla. They are expressed throughout the body, with a diversity of functions including axon guidance, synapse development and plasticity, cell proliferation and migration, dendritic arborization, and neuronal polarization. Class 5 semaphorins, Sema5A and Sema5B, are highly similar transmembrane proteins, functioning in developmental axon guidance and synapse plasticity. Sema5A is genetically-linked to Autism Spectrum Disorder (ASD) and its expression is decreased in people with ASD. However, its function in synapse plasticity is poorly understood. Our study examines the role of Sema5A in synapse elimination and the regulation of class 5 semaphorins by neural activity, specifically long-term potentiation (LTP) and long-term depression (LTD). We demonstrate that Sema5A, like Sema5B mediates synapse elimination of hippocampal neurons. Furthermore, we show that the expression of both class 5 semaphorins is up-regulated by LTD and that LTD enhances the membrane localization of Sema5A. As LTD and Sema5A were independently found to mediate synapse elimination and as we determined that Sema5A is up-regulated by LTD, we tested the hypothesis that LTD-mediates synapse elimination through Sema5A up-regulation. However, we discovered that Sema5A is not required for LTD-mediated synapse elimination and therefore likely functions to eliminate synapses through a separate pathway.
Progranulin (PGRN) is a multi-functional, secreted growth factor expressed in a variety of tissues throughout the body. In the central nervous system (CNS), PGRN is expressed in microglia as well as in a number of neuronal populations and has been shown to promote neuronal survival, enhance neurite outgrowth and regulate inflammation and development. Mutations in the progranulin (GRN) gene have been identified as a major cause of autosomal dominant frontotemporal dementia (FTD) with tau-negative inclusions. The majority of GRN mutations result in the production of a null allele and reduced PGRN expression. However, the normal functions of PGRN in the CNS remain poorly understood. Our study examines the secretion characteristics of PGRN in neurons. To study the secretion of PGRN from axons and dendrites, we have fused a pH-sensitive optical reporter of exocytosis, superecliptic pHluorin, to PGRN (PGRN-SEP). We demonstrate that activity enhances the secretion of PGRN from axons and dendrites with different temporal profiles of secretion. We show, using calcium blockers and calcium-free media, that activity-mediated secretion of PGRN requires Ca²⁺ entry via voltage-gated calcium channels (VGCC). We postulate that activity-dependent secretion of PGRN may enhance the formation and maturation of synapses as treatment of hippocampal neurons with recombinant PGRN results in an increase in synapse density.
Cadherins and Neuroligins (NLs) are two of the most extensively studied cell adhesion molecules (CAMs) at synapses and have previously been shown to localize to synapses and exert a key role during their development. Despite this, their spatial and functional relationship with respect to one another has not been studied to date. In the present study, we examine the spatial and functional relationship of cadherin and NL isoforms at glutamatergic and GABAergic synapses in cultured hippocampal neurons. Analysis of the synaptic distribution of N-cadherin and NL1 and NL2 in hippocampal cultures, confirm previous studies demonstrating the enrichment of NL2 at GABAergic synapses and enrichment of NL1 and N-cadherin at glutamatergic synapses. We have also observed subsets of GABAergic synapses that express both N-cadherin and NL2 as well as glutamatergic synapses that only express either NL1 or N-cadherin. These groups of glutmatergic and GABAergic synapses may represent a specific subtype of synapse, or may reflect the differential localization of these adhesion molecules during synapse formation. Moreover, using a combination of overexpression and knockdown analysis we demonstrate that NL1 and N-cadherin promote the formation of synapses, in part, by a common pathway. Indeed, knocking down these proteins individually results in approximately 50% reduction in glutamatergic synapse density with a similar reduction upon combined knockdown. In addition, functional compensation assays demonstrate that NL1 expression can fully rescue synapse loss that is due to knockdown of N-cadherin expression. Furthermore, N-cadherin expression can partially rescue synapse loss that is due to knockdown of NL1 expression. Together this work demonstrates that these two cell adhesion proteins act in concert to regulate excitatory synapse formation. Specifically, we show that N-cadherin acts upstream of NL1 to promote synapse formation and that NL1 is a limiting factor in this pathway.