Relevant Degree Programs
Graduate Student Supervision
Doctoral Student Supervision (Jan 2008 - Nov 2019)
Retinitis pigmentosa (RP) is an inherited retinal degeneration (RD) that leads to blindness for which no treatment is available. RP is frequently caused by mutations in rhodopsin; in some animal models, RD is exacerbated by light. Valproic acid (VPA) is a proposed treatment for RP and other neurodegenerative disorders. A phase II clinical for RP was conducted in parallel of this thesis. However, the therapeutic mechanism is unclear, with minimal research supporting its use in RP.We investigated the effects of VPA on X. laevis models of RP expressing human P23H, T17M, T4K, and Q344ter rhodopsins, which are associated with RP in humans. VPA ameliorated RD associated with P23H rhodopsin and promoted clearing of mutant rhodopsin from photoreceptors. The effect was equal to that of dark-rearing, with no additive effect observed. Rescue of visual function was confirmed by electroretinography. Contrastingly, VPA exacerbated RD caused by T17M rhodopsin in light, but had no effect in darkness. Effects in T4K and Q344ter rhodopsin models were also negative. These effects of VPA were paralleled by treatment with three additional histone deacetylase inhibitors (HDACi’s), but not other antipsychotics, chemical chaperones, or VPA structural analogs. In wildtype retinas, VPA treatment increased histone H3 acetylation. Additionally, electron microscopy showed increased autophagosomes in rod inner segments with HDACi treatment, potentially linking the therapeutic effects in P23H rhodopsin animals and negative effects in other models with autophagy. Our results suggest that the success or failure of VPA treatment will be dependent on genotype and that HDACi treatment is contraindicated for some RP cases.
Retinitis pigmentosa (RP) is a genetic neurodegenerative disorder that causes progressive cell death of the rod and cone photoreceptors, eventually leading to blindness. The light-sensitive protein in rods, rhodopsin, is composed of the chromophore 11-cis retinal and the protein rod opsin. Mutations in the rhodopsin gene are common causes of RP. Autophagy is a lysosomal-turnover pathway for degrading dysfunctional proteins, organelles or other cellular components that is necessary for maintaining cellular homeostasis. We observed an increase of autophagy structures in rods expressing the misfolding-prone rhodopsin mutant P23H (Bogéa et al. 2015). However, the role autophagy plays in RP is not clearly understood. To examine the role of autophagy in normal and diseased rods, I generated transgenic Xenopus laevis tadpoles expressing the autophagy reporter mRFP-eGFP-LC3.My results demonstrate that the autophagy process lasts for about 34 h in normal rods. Early autophagic structures persist for 6 to 8 h before fusing with lysosomes and acidification; acidified autolysosomes persist for about 28 h before complete digestion. Autophagy in normal rods is diurnally regulated, with more autophagic structures generated in light and fewer in darkness; this regulation is non-circadian. Autophagy also increased in rods co-expressing P23H rhodopsin. The rhodopsin chromophore, a pharmacological chaperone for rhodopsin, absorbs photons to initiate phototransduction, and is consumed in this process; it also promotes proper rhodopsin folding. To determine whether increased autophagy in light-exposed normal rods is caused by increased misfolding of wildtype rhodopsin due to lack of chromophore, I used CRISPR/Cas9 to knock out the gene RPE65, which is essential for chromophore biosynthesis. I observed that eliminating chromophore does not promote autophagy in dark-reared rods, but prevents induction of autophagy in light-exposed rods. This combination of outcomes suggests that, although rhodopsin misfolding can induce autophagy, light-induced autophagy is not due to misfolding of rhodopsin, but rather due to phototransduction.Further, I found that a group of compounds called histone deacetylase (HDAC) inhibitors, valproic acid (VPA), sodium butyrate (NaBu) and CI-994, consistently promote autophagy in rods; these compounds were previously demonstrated to ameliorate retinal degeneration associated with P23H rhodopsin (Vent-schmidt et al. 2017).
Master's Student Supervision (2010 - 2018)
Xenopus laevis is a commonly used research subject for retinal physiology and cell biology studies, but its utility is limited by the lack of a robust technology for generation of knock-out (KO) or knock-down (KD) phenotypes. However, new genome manipulation techniques involving CRISPR/Cas9 offer an opportunity for generating gene KOs in X. laevis. RNA-guided Cas9 endonuclease introduces double-stranded DNA breaks into the genome, which are either repaired by error-prone non-homologous-end joining (NHEJ), facilitating indel generation, or by less error-prone homology-directed repair (HDR), facilitating insertion of specific sequences. Rhodopsin was targeted for editing as the expected phenotypes, missing/malformed rod photoreceptor outer segments and lower rhodopsin content, are easily assayed. RNA and transgene methods for CRISPR/Cas9-mediated rhodopsin KOs and knock-ins (KI) in rod photoreceptors of X. laevis were tested, and an RNA injection protocol was developed and optimized. KOs were generated by in vitro transcription and microinjection of Cas9 mRNA, eGFP mRNA, and sgRNAs into in vitro fertilized eggs. Cas9 transgene cassettes were built and tested but editing attempts were unsuccessful. Indel mutations were identified by direct sequencing of PCR products and further characterized by sequencing individual clones. The extent of rhodopsin KO was quantified in 14 post-fertilization day-old tadpoles by anti-rod opsin dot blot assay of retinal extracts, and retinal phenotypes were assessed by cryosectioning and immunolabeling contralateral eyes for confocal microscopy. HDR-mediated KIs were generated by co-injection of a DNA repair fragment, with sufficient homology to the genomic region surrounding predicted dsDNA break-site. Heterologous expression of KIs was confirmed by immunohistochemistry. Delivery of Cas9 by RNA injection can produce high frequency homozygous and heterozygous KOs in X. laevis, permitting analysis in the first generation. I was able to obtain extensive KD generating very severe retinal degeneration phenotypes, and germline transmission of Cas9-mediated indels was confirmed. However, KO was never complete. Sequencing results indicate that first generation animals are chimeric containing many independently derived indels. HDR-mediated KI techniques proved possible, but low in efficiency. These techniques significantly advance the utility of X. laevis as an experimental subject for cell biology and physiology studies.
Protocadherin-21 (pcdh-21) is a transmembrane protein concentrated at nascent disks in mouse photoreceptors and thought to regulate disk synthesis. PCDH-21 mutations are associated with retinal degenerative diseases. Pcdh-21 undergoes proteolytic cleavage that may be essential for disk synthesis. In mice, Pcdh-21 interacts with prominin-1 (prom-1) and their interaction may be required for their localization and function in disk synthesis.To compare pcdh-21 localization across species, we performed immunofluorescence microscopy using an antibody raised against the N-terminus of X.laevis pcdh-21 (xpcdh-21). In rods and cones of all species, pcdh-21 was localized to nascent disks at the base of the outer segment, suggesting a conserved role in disk assembly. However, in contrast with the idea that pcdh-21 localizes only to the basal outer segment, pcdh-21 was localized to other outer segment regions, and this localization was different across cell types and species, suggesting that pcdh-21 has cell type- and species- specific structural roles. Pcdh-21 was restricted to open disk rims in X.laevis cones. Prom-1, an interacting partner of pcdh-21 in mice, shows identical labeling at the open disk rims. Pcdh-21 and prom-1 may therefore interact to maintain open disk structure. Immunoblots showed that proteolytic cleavage of pcdh-21 may be unique to mice. In X.laevis rods, pcdh-21 labeling in the nascent disks did not vary with disk synthesis rate.We attempted to inhibit pcdh-21 function using a dominant negative approach. Full length pcdh-21 (FL) and deletion constructs consists of mouse (mpcdh-21) and xpcdh-21 were overexpressed in X.laevis rods. Retinal degeneration and disk defects were only observed in retinas overexpressing mpcdh-21 FL. Mpcdh-21 FL was retained in the ER, caused abnormal ER structure, and was not cleaved in X. laevis retinas. Xpcdh-21 variants were correctly localized and did not cause retinal degeneration.This study illustrated that pcdh-21 localization, processing and properties may not be conserved across species. Differences in pcdh-21 localization may reflect differences in disk synthesis mechanisms or disk ultrastructure. However, the conserved association of pcdh-21 and prom-1 with open disk rims and nascent disks suggests that they may form a complex involved in regulating disk synthesis and/ or in maintaining disk structure.