Pauline Johnson

 
Prospective Graduate Students / Postdocs

This faculty member is currently not looking for graduate students or Postdoctoral Fellows. Please do not contact the faculty member with any such requests.

Professor

Research Classification

Diseases of the Immune System

Research Interests

Immunology
Inflammation
Immune-cancer interactions
Innate immunity
Macrophages and myeloid cells

Relevant Degree Programs

Affiliations to Research Centres, Institutes & Clusters

 
 

Research Methodology

Flow cytometry
Disease models
in vivo and in vitro analysis

Publications

  • Splenic erythroid progenitors decrease TNF‐α production by macrophages and reduce systemic inflammation in a mouse model of T cell‐induced colitis (2021)
    European Journal of Immunology,
  • Regulation of CD71+TER119+ erythroid progenitor cells by CD45 (2020)
    Experimental Hematology, 86, 53--66.e1
  • RNA sequencing and lipidomic analysis of alveolar macrophages from normal and CD44 deficient mice (2019)
  • ATG Genes Influence the Virulence of Cryptococcus neoformans through Contributions beyond Core Autophagy Functions. (2018)
    Infection and immunity,
  • CD44-mediated hyaluronan binding marks proliferating hematopoietic progenitor cells and promotes bone marrow engraftment (2018)
    PLOS ONE,
  • CD44-mediated hyaluronan binding marks proliferating hematopoietic progenitor cells and promotes bone marrow engraftment. (2018)
    PloS one,
  • Hyaluronan and Its Interactions With Immune Cells in the Healthy and Inflamed Lung. (2018)
    Frontiers in immunology,
  • Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells (2018)
    European Journal of Immunology,
  • Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells. (2018)
    European journal of immunology,
  • Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement. (2018)
    Cell,
  • The survival of fetal and bone marrow monocyte-derived alveolar macrophages is promoted by CD44 and its interaction with hyaluronan. (2017)
    Mucosal immunology,
  • CD45 phosphatase is crucial for human and murine acute myeloid leukemia maintenance through its localization in lipid rafts (2016)
    Oncotarget, 7 (40), 64785
  • CD45 regulates GM-CSF, retinoic acid and T-cell homing in intestinal inflammation (2016)
    Mucosal immunology, 9 (6), 1514
  • Endotoxin free hyaluronan and hyaluronan fragments do not stimulate TNF-$α$, interleukin-12 or upregulate co-stimulatory molecules in dendritic cells or macrophages (2016)
    Scientific reports, 6, 36928
  • Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow (2016)
    JoVE (Journal of Visualized Experiments), (112), e54194
  • Hyaluronan binding identifies a functionally distinct alveolar macrophage--like population in bone marrow--derived dendritic cell cultures (2015)
    The Journal of Immunology, 195 (2), 632--642
  • The where, when, how, and why of hyaluronan binding by immune cells (2015)
    Frontiers in immunology, 6, 150
  • Arginase activity in alternatively activated macrophages protects PI3Kp110$δ$ deficient mice from dextran sodium sulfate induced intestinal inflammation (2014)
    European journal of immunology, 44 (11), 3353--3367
  • Innate immune cell CD45 regulates lymphopenia-induced T cell proliferation (2014)
    The Journal of Immunology, 193 (6), 2831--2842
  • Glycoproteins with Monoclonal Antibody Affinity Columns (2013)
    Hybridoma Technology in the Biosciences and Medicine, , 163
  • Temporal stability of the mouse gut microbiota in relation to innate and adaptive immunity (2013)
    Environmental microbiology reports, 5 (2), 200--210
  • CD45 (PTPRC) (2012)
    Encyclopedia of Signaling Molecules, , 328--334
  • Optical Methods (2012)
    The Enzymes of Biological Membranes: Volume 1 Membrane Structure and Dynamics, , 421
  • The tyrosine phosphatase CD45 regulates lymphopenia-induced T cell proliferation (47.15) (2012)
  • CD44-mediated elongated T cell spreading requires Pyk2 activation by Src family kinases, extracellular calcium, phospholipase C and phosphatidylinositol-3 kinase (2011)
    Cellular signalling, 23 (5), 812--819
  • Differential use of chondroitin sulfate to regulate hyaluronan binding by receptor CD44 in inflammatory and interleukin 4-activated macrophages (2011)
    Journal of Biological Chemistry, 286 (22), 19179--19190
  • Host defense peptide LL-37 selectively reduces proinflammatory macrophage responses (2011)
    The Journal of Immunology, 186 (9), 5497--5505
  • Hyaluronan binding identifies the most proliferative activated and memory T cells (2011)
    European journal of immunology, 41 (4), 1108--1119
  • The Suv39H1 methyltransferase inhibitor chaetocin causes induction of integrated HIV-1 without producing a T cell response (2011)
    FEBS letters, 585 (22), 3549--3554
  • CD44 interacts directly with Lck in a zinc-dependent manner (2010)
    Molecular immunology, 47 (10), 1882--1889
  • CD45 regulates migration, proliferation, and progression of double negative 1 thymocytes (2010)
    The Journal of Immunology, 185 (4), 2059--2070
  • Modulation of immune cell signalling by the leukocyte common tyrosine phosphatase, CD45 (2010)
    Cellular signalling, 22 (3), 339--348
  • Y-box binding protein-1 induces the expression of CD44 and CD49f leading to enhanced self-renewal, mammosphere growth, and drug resistance (2010)
    Cancer research, 70 (7), 2840--2851
  • CD44 and its role in inflammation and inflammatory diseases (2009)
    Inflammation & Allergy-Drug Targets (Formerly Current Drug Targets-Inflammation & Allergy), 8 (3), 208--220
  • CD45-mediated fodrin cleavage during galectin-1 T cell death promotes phagocytic clearance of dying cells (2009)
    The Journal of Immunology, 182 (11), 7001--7008
  • Tyrosine phosphorylation in immune cells: direct and indirect effects on toll-like receptor-induced proinflammatory cytokine production (2009)
    Critical Reviews� in Immunology, 29 (4)
  • CD45 Down-Regulates Lck-Mediated CD44 (2008)
    J Immunol, 181, 7033--7043
  • CD45 down-regulates Lck-mediated CD44 signaling and modulates actin rearrangement in T cells (2008)
    The Journal of Immunology, 181 (10), 7033--7043
  • CD45 regulates TLR-induced proinflammatory cytokine and IFN-$β$ secretion in dendritic cells (2008)
    The Journal of Immunology, 180 (12), 8020--8029
  • CD45 Regulates TLR-Induced Proinflammatory Cytokine and IFN-Secretion in Dendritic Cells (2008)
  • Hyaluronan induces cell death in activated T cells through CD44 (2008)
    The Journal of Immunology, 181 (10), 7044--7054
  • Differential function of PTP$α$ and PTP$α$ Y789F in T cells and regulation of PTP$α$ phosphorylation at Tyr-789 by CD45 (2007)
    Journal of Biological Chemistry, 282 (29), 20925--20932
  • Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death (2006)
    The Journal of Immunology, 176 (2), 778--789
  • H2-M3-restricted T cells participate in the priming of antigen-specific CD4+ T cells (2006)
    The Journal of Immunology, 177 (8), 5098--5104
  • Use of CD44 by CD4+ Th1 and Th2 lymphocytes to roll and adhere (2006)
    Blood, 107 (12), 4798--4806
  • Chondroitin sulfate addition to CD44H negatively regulates hyaluronan binding (2005)
    Biochemical and biophysical research communications, 334 (2), 306--312
  • Expression of CD45 lacking the catalytic protein tyrosine phosphatase domain modulates Lck phosphorylation and T cell activation (2005)
    Journal of Biological Chemistry, 280 (14), 14318--14324
  • Expression of N-acetylglucosamine 6-O-sulfotransferases (GlcNAc6STs)-1 and-4 in human monocytes: GlcNAc6ST-1 is implicated in the generation of the 6-sulfo N-acetyllactosamine/Lewis x epitope on CD44 and is induced by TNF-$α$ (2005)
    Glycobiology, 15 (7), 7C--13C
  • Regulation of hyaluronan binding by F-actin and colocalization of CD44 and phosphorylated ezrin/radixin/moesin (ERM) proteins in myeloid cells (2005)
    Experimental cell research, 303 (2), 400--414
  • Chondroitin sulfate chains on CD44 negatively regulate its ability to bind hyaluronan (2004)
    GLYCOBIOLOGY, 14 (11), 1108--1108
  • Role of CD44 and Hyaluronan in Neutrophil (2004)
    J Immunol, 173, 7594--7601
  • Role of CD44 and hyaluronan in neutrophil recruitment (2004)
    The Journal of Immunology, 173 (12), 7594--7601
  • Subdomain X of the kinase domain of Lck binds CD45 and facilitates dephosphorylation (2004)
    Journal of Biological Chemistry, 279 (5), 3455--3462
  • A cell death pathway induced by antibody-mediated cross-linking of CD45 on lymphocytes (2003)
    Critical Reviews� in Immunology, 23 (5-6)
  • Proteins and Proteomics Cumulative Contents (2003)
    Biochimica et Biophysica Acta, 1650, 147
  • The noncatalytic domains of Lck regulate its dephosphorylation by CD45 (2003)
    Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1650 (1-2), 40--49
  • Apoptosis mediated through CD45 is independent of its phosphatase activity and association with leukocyte phosphatase-associated phosphoprotein (2002)
    The Journal of Immunology, 168 (12), 6084--6089
  • CELLULAR IMMUNOLOGY AND IMMUNE REGULATION-Apoptosis Mediated Through CD45 Is Independent of Its Phosphatase Activity and Association with Leukocyte Phosphatase-Associated Phosphoprotein (2002)
    Journal of Immunology, 168 (12), 6084--6089
  • TNF-$α$ increases the carbohydrate sulfation of CD44: induction of 6-sulfo N-acetyl lactosamine on N-and O-linked glycans (2002)
    Glycobiology, 12 (10), 613--622
  • CD44-initiated cell spreading induces Pyk2 phosphorylation, is mediated by Src family kinases, and is negatively regulated by CD45 (2001)
    Journal of Biological Chemistry, 276 (31), 28767--28773
  • CD45 is a JAK phosphatase and negatively regulates cytokine receptor signalling (2001)
    Nature, 409 (6818), 349
  • MECHANISMS OF SIGNAL TRANSDUCTION-CD44-initiated cell spreading induces Pyk2 phosphorylation, is mediated by Src family kinases, and is negatively regulated by CD45. (2001)
    Journal of Biological Chemistry, 276 (31), 28767--28773
  • Role of sulfation in CD44-mediated hyaluronan binding induced by inflammatory mediators in human CD14+ peripheral blood monocytes (2001)
    The Journal of Immunology, 167 (9), 5367--5374
  • A role for the cell adhesion molecule CD44 and sulfation in leukocyte--endothelial cell adhesion during an inflammatory response? (2000)
    Biochemical pharmacology, 59 (5), 455--465
  • CD45 regulates CD CD44-initiated cytoskeletal changes and cell spreading in T cells (2000)
    FASEB JOURNAL, 14 (6), A1143--A1143
  • Characterization of the interaction between the tyrosine phosphatase, CD45, and its substrate, p56lck (2000)
    FASEB JOURNAL, 14 (6), A1174--A1174
  • Characterization of TNF-alpha induced sulfation of CD44 (2000)
    FASEB JOURNAL, 14 (6), A1142--A1142
  • Determination of CD45 tyrosine phosphatase activity in T lymphocytes (2000)
    T Cell Protocols, , 289--300
  • Regulation of hyaluronan binding by CD44 on human peripheral blood monocytes. (2000)
    FASEB JOURNAL, 14 (6), A1142--A1142
  • Stable interdomain interaction within the cytoplasmic domain of CD45 increases enzyme stability (2000)
    Biochemical and biophysical research communications, 271 (2), 292--298
  • Leukocyte adhesion mediated by sulfated CD44 (1999)
    Biomedicine & Pharmacotherapy, 8 (53), 393
  • Analysis of CD44 interactions with hyaluronan in murine L cell fibroblasts deficient in glycosaminoglycan synthesis: a role for chondroitin sulfate (1998)
    Journal of Cell Science, 111 (7), 1021--1029
  • CD45: A family of leukocyte-specific cell surface glycoproteins (1998)
    Weir's handbook of experimental immunology, 2, 62--1
  • Characterization of Recombinant CD45 Cytoplasmic Domain Proteins EVIDENCE FOR INTRAMOLECULAR AND INTERMOLECULAR INTERACTIONS (1998)
    Journal of Biological Chemistry, 273 (28), 17839--17845
  • Chimeric CD4/CD44 molecules associate with CD44 via the transmembrane region and reduce hyaluronan binding in T cell lines (1998)
    European journal of immunology, 28 (6), 1745--1754
  • PROTEIN CHEMISTRY AND STRUCTURE-Characterization of recombinant CD45 cytoplasmic domain proteins. Evidence for intramolecular and intermolecular interactions. (1998)
    Journal of Biological Chemistry, 273 (28), 17839--17845
  • Regulation of CD44 mediated adhesion by sulfation (1998)
    MOLECULAR BIOLOGY OF THE CELL, 9, 198A--198A
  • The association between CD20 and Src-family tyrosine kinases requires an additional factor (1998)
    Molecular immunology, 35 (4), 207--214
  • TNF-$α$ induction of CD44-mediated leukocyte adhesion by sulfation (1998)
    Science, 282 (5390), 941--943
  • CD45 and RPTP$α$ display different protein tyrosine phosphatase activities in T lymphocytes (1997)
    Biochemical Journal, 327 (3), 867--876
  • Demonstration of a Direct Interaction between p56 and the Cytoplasmic Domain of CD45 in Vitro (1996)
    Journal of Biological Chemistry, 271 (3), 1295--1300
  • Increase in the specific activity of p50csk in proliferating T cells correlates with decreased specific activity of p56lck and p59fyn and reduced phosphorylation of CD3 subunits (1996)
    Molecular immunology, 33 (6), 531--540
  • Regulation of hyaluronan binding to CD44 by chondroitin sulfate and N-glycosylation. (1996)
    MOLECULAR BIOLOGY OF THE CELL, 7, 374--374
  • Association of 75/80-kDa Phosphoproteins and the Tyrosine Kinases Lyn, Fyn, and Lck with the B Cell Molecule CD20 EVIDENCE AGAINST INVOLVEMENT OF THE CYTOPLASMIC REGIONS OF CD20 (1995)
    Journal of Biological Chemistry, 270 (38), 22632--22638
  • Characterization of the interaction between CD45 and CD45-AP (1995)
    Journal of Biological Chemistry, 270 (36), 21151--21157
  • Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells (1995)
    European journal of immunology, 25 (12), 3370--3374
  • Non-radioactive method to measure CD45 protein tyrosine phosphatase activity isolated directly from cells (1995)
    Journal of immunological methods, 179 (2), 177--185
  • Point mutation in the second phosphatase domain of CD45 abrogates tyrosine phosphatase activity (1995)
    Biochemical and biophysical research communications, 206 (1), 302--309
  • Isoforms of the transmembrane tyrosine phosphatase CD45 differentially affect T cell recognition (1994)
    Immunity, 1 (2), 109--119
  • Protein tyrosine phosphorylation in Mycobacterium tuberculosis (1994)
    FEMS microbiology letters, 124 (2), 203--207
  • Specific CD45 isoforms differentially regulate T cell receptor signaling. (1994)
    The EMBO journal, 13 (4), 798--807
  • Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) zeta subunit: regulation of TCR-associated protein tyrosine kinase activity by TCR zeta. (1993)
    Molecular and cellular biology, 13 (9), 5771--5780
  • Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. (1992)
    The Journal of Immunology, 148 (1), 19--28
  • Mutational analysis of CD45. A leukocyte-specific protein tyrosine phosphatase. (1992)
    Journal of Biological Chemistry, 267 (12), 8035--8041
  • Regulation and structure-function relationship of CD45 (1992)
  • Structure and function of CD45: a leukocyte-specific protein tyrosine phosphatase (1992)
    Mechanisms of Lymphocyte Activation and Immune Regulation IV, , 29--37
  • CD45-A LEUKOCYTE-SPECIFIC PROTEIN TYROSINE-PHOSPHATASE (1991)
    FASEB JOURNAL, 5 (4), A784--A784
  • CD45: a leukocyte-specific member of the protein tyrosine phosphatase family (1991)
    Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1095 (1), 46--56
  • Differential expression of the alternatively spliced exons of murine CD45 in Th1 and Th2 cell clones (1991)
    European journal of immunology, 21 (1), 17--22
  • Changes in CD45 isoform expression accompany antigen-induced murine T-cell activation (1989)
    Proceedings of the National Academy of Sciences, 86 (17), 6734--6738
  • Expression of CD45 alters phosphorylation of the lck-encoded tyrosine protein kinase in murine lymphoma T-cell lines (1989)
    Proceedings of the National Academy of Sciences, 86 (22), 8959--8963
  • Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. (1989)
    Journal of Experimental Medicine, 169 (3), 1179--1184
  • CD8B, the human equivalent of the mouse Ly-3 gene is localized on chromosome 2 (1988)
    Immunogenetics, 27 (1), 70--72
  • Immunoglobulin-related structures associated with vertebrate cell surfaces (1988)
    The T-Cell Receptors, , 53--87
  • A human homolog of the mouse CD8 molecule, Lyt-3: genomic sequence and expression (1987)
    Immunogenetics, 26 (3), 174--177
  • CD8 THE HUMAN EQUIVALENT OF THE MOUSE LYT-3 GENE IS ASSIGNED TO CHROMOSOME-2 (1987)
    CYTOGENETICS AND CELL GENETICS, 46 (1-4), 698--698
  • T4. 5 Identification of human Lyt 3 at the genomic level (1987)
    Leucocyte Typing III: White Cell Differentiation Antigens, , 215
  • Human Hybridomas and Monoclonal Antibodies (1986)
  • Purification and sequence of the rat CDS antigen, a cytotoxic T-cell marker (1986)
  • Striking similarities between antigen receptor J pieces and sequence in the second chain of the murine CD8 antigen (1986)
    Nature, 323 (6083), 74
  • I. HISTORICAL BACKGROUND (1985)
    The Enzymes of Biological Membranes: Membrane structure and dynamics, 1, 421
  • Optical methods for measuring the rotational diffusion of membrane proteins (1985)
    Spectroscopy and the dynamics of Molecular Biological Systems, PM Bayley and RE Dale, eds, , 95--118
  • Purification of membrane glycoproteins with monoclonal antibody affinity columns (1985)
    Hybridoma Technology in the Biosciences and Medicine, , 163--175
  • Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes. (1985)
    The EMBO journal, 4 (10), 2539--2545
  • Rotational diffusion of membrane proteins optical methods (1985)
    The Enzymes of Biological Membranes, , 421--439
  • High affinity epidermal growth factor receptors on the surface of A431 cells have restricted lateral diffusion. (1984)
    The EMBO journal, 3 (8), 1843--1847
  • Carbocyanine dyes used as fluorescent triplet probes for measuring slow rotational diffusion of lipids in membranes (1983)
    FEBS letters, 153 (2), 391--394
  • Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy (1983)
    FEBS letters, 161 (1), 21--27
  • Changes in the properties of the surface membrane of Schistosoma mansoni during growth as measured by fluorescence recovery after photobleaching (1982)
    FEBS letters, 141 (1), 132--135
  • Fluorescent triplet probes for measuring the rotational diffusion of membrane proteins (1982)
    Biochemical Journal, 203 (1), 313--321
  • Depolarization of fluorescence depletion: A microscopic method for measuring rotational diffusion of membrane proteins on the surface of a single cell (1981)
    FEBS letters, 132 (2), 252--256
 
 

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