Professor
Research Classification
Research Interests
Structural Biology
cryo-electron microscopy
drug discovery
Cryo-electron tomography
Antibody design
drug discovery
Protein therapeutics
COVID-19 therapeutic design
Infectious disease
Artificial Intelligence
Machine Learning
Relevant Thesis-Based Degree Programs
Affiliations to Research Centres, Institutes & Clusters
Research Options
I am interested in and conduct interdisciplinary research.
Research Methodology
electron microscopes operating at cryogenic temperatures
Publications
- AAA ATPase protein–protein interactions as therapeutic targets in cancer (2024)
Current Opinion in Cell Biology, 86 - Altered receptor binding, antibody evasion and retention of T cell recognition by the SARS-CoV-2 XBB.1.5 spike protein (2024)
Nature Communications, 15 (1) - Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM (2024)
Nature Structural and Molecular Biology, 31 (3), 424-435 - Community recommendations on cryoEM data archiving and validation (2024)
IUCrJ, 11 (Pt 2), 140-151 - SARS-CoV-2 Variants Omicron BA.4/5 and XBB.1.5 Significantly Escape T Cell Recognition in Solid-organ Transplant Recipients Vaccinated Against the Ancestral Strain (2024)
Transplantation, 108 (4), E49-E62 - Structural biology in the age of AI (2024)
Nature Methods, 21 (1), 18-19 - Allosteric modulation of ryanodine receptor RyR1 by nucleotide derivatives (2023)
Structure, 31 (7), 790-800.e4 - Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold (2023)
Proceedings of the National Academy of Sciences of the United States of America, 120 (37) - Learning from Gobind Khorana: “The difficult we do today, the impossible tomorrow” (2023)
Biophysical Reviews, 15 (1), 89-91 - Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein (2023)
Cell Reports, 42 (1) - A paradigm shift in structural biology (2022)
Nature Methods, 19 (1), 20-23 - Comparison of side-chain dispersion in protein structures determined by cryo-EM and X-ray crystallography (2022)
IUCrJ, 9, 98-103 - Cryo-EM at ACA 2022 (2022)
IUCrJ, 9 (6), 713-714 - Imaging Cellular Architecture in Three Dimensions Through Electron Microscopy (2022)
Encyclopedia of Cell Biology: Volume 1-6, Second Edition, 2, 51-60 - Introduction: Cryo-EM in Biology and Materials Research (2022)
Chemical Reviews, 122 (17), 13881-13882 - Potent and broad neutralization of SARS-CoV-2 variants of concern (VOCs) including omicron sub-lineages BA.1 and BA.2 by biparatopic human VH domains (2022)
iScience, 25 (8) - SARS-CoV-2 Omicron variant: Antibody evasion and cryo-EM structure of spike protein–ACE2 complex (2022)
Science, 375 (6582), 760-764 - SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization (2022)
Nature Communications, 13 (1) - Structural and biochemical rationale for enhanced spike protein fitness in delta and kappa SARS-CoV-2 variants (2022)
Nature Communications, 13 (1) - Structure and activity of human TMPRSS2 protease implicated in SARS-CoV-2 activation (2022)
Nature Chemical Biology, 18 (9), 963-971 - Three-Dimensional Visualization of Viral Structure, Entry, and Replication Underlying the Spread of SARS-CoV-2 (2022)
Chemical Reviews, 122 (17), 14066-14084 - A molecular mechanism for the generation of Ligand-dependent differential outputs by the epidermal growth factor receptor (2021)
eLife, 10 - AAA+ ATPase p97/VCP mutants and inhibitor binding disrupt inter-domain coupling and subsequent allosteric activation (2021)
Journal of Biological Chemistry, 297 (4) - Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies (2021)
PLoS Biology, 19 (4) - Glycan reactive anti-HIV-1 antibodies bind the SARS-CoV-2 spike protein but do not block viral entry (2021)
Scientific Reports, 11 (1) - Malaria parasites use a soluble Rhoph complex for erythrocyte invasion and an integral form for nutrient uptake (2021)
eLife, 10, 1-24 - New insights into Raf regulation from structural analyses (2021)
Current Opinion in Structural Biology, 71, 223-231 - Structural analysis of receptor binding domain mutations in SARS-CoV-2 variants of concern that modulate ACE2 and antibody binding (2021)
Cell Reports, 37 (12) - 1.8 Å resolution structure of β-galactosidase with a 200 kV CRYO ARM electron microscope (2020)
IUCrJ, 7, 639-643 - Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer (2020)
Journal of Biological Chemistry, 295 (4), 1105-1119 - COVID-19 and cryo-EM (2020)
IUCrJ, 7, 575-576 - Data management pipeline at the national cryo-EM facility (2020)
Microscopy and Microanalysis - Data management pipeline at the national cryo-EM facility (2020)
Microscopy and Microanalysis - High Potency of a Bivalent Human VH Domain in SARS-CoV-2 Animal Models (2020)
Cell, 183 (2), 429-441.e16 - Host membrane lipids are trafficked to membranes of intravacuolar bacterium Ehrlichia chaffeensis (2020)
Proceedings of the National Academy of Sciences of the United States of America, 117 (14), 8032-8043 - Plasmodium vivax and human hexokinases share similar active sites but display distinct quaternary architectures (2020)
IUCrJ, 7, 453-461 - Visualizing Cancer (2020)
Cancer Cell, 38 (6), 753-756 - A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex (2019)
Cell Reports, 27 (2), 387-399.e7 - Cryo-electron microscopy instrumentation and techniques for life sciences and materials science (2019)
MRS Bulletin, 44 (12), 929-934 - Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases (2019)
Science, 366 (6461), 109-115 - Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9 (2019)
Nature Structural and Molecular Biology, 26 (8), 679-685 - Deep-learning-assisted volume visualization (2019)
IEEE Transactions on Visualization and Computer Graphics, 25 (2), 1378-1391 - Frontiers in Cryo Electron Microscopy of Complex Macromolecular Assemblies (2019)
Annual Review of Biomedical Engineering, 21, 395-415 - Semi-automated 3D segmentation of human skeletal muscle using Focused Ion Beam-Scanning Electron Microscopic images (2019)
Journal of Structural Biology, 207 (1), 1-11 - Structure of the primed state of the ATPase domain of chromatin remodeling factor ISWI bound to the nucleosome (2019)
Nucleic Acids Research, 47 (17), 9400-9409 - Atomic Resolution Cryo-EM Structure of β-Galactosidase (2018)
Structure, 26 (6), 848-856.e3 - Cryo-EM structure of human rhodopsin bound to an inhibitory G protein (2018)
Nature, 558 (7711), 553-558 - Erratum to: Cryo-EM structure of human rhodopsin bound to an inhibitory G protein (Nature, (2018), 558, 7711, (553-558), 10.1038/s41586-018-0215-y) (2018)
Nature, 561 (7724), E44 - Erratum: Power Grid Protection of the Muscle Mitochondrial Reticulum (Cell Reports (2017) 19(3) (487–496)(S2211124717304242)(10.1016/j.celrep.2017.03.063)) (2018)
Cell Reports, 23 (9), 2832 - Microbiology catches the cryo-EM bug (2018)
Current Opinion in Microbiology, 43, 199-207 - Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs (2018)
eLife, 7 - Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N (2018)
Science, 359 (6373), 339-343 - Broadly protective murine monoclonal antibodies against influenza B virus target highly conserved neuraminidase epitopes (2017)
Nature Microbiology, 2 (10), 1415-1424 - Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex (2017)
Cell, 171 (2), 414-426.e12 - Cryo-EM: beyond the microscope (2017)
Current Opinion in Structural Biology, 46, 71-78 - Cutting Edge: Building bridges between cellular and molecular structural biology (2017)
eLife, 6 - Power Grid Protection of the Muscle Mitochondrial Reticulum (2017)
Cell Reports, 19 (3), 487-496 - 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition (2016)
Science, 351 (6275), 871-875 - Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery (2016)
Cell, 165 (7), 1698-1707 - Cryo-electron microscopy structures of chimeric hemagglutinin displayed on a universal influenza vaccine candidate (2016)
mBio, 7 (2) - Cryo-EM analysis of the conformational landscape of human P-glycoprotein (ABCB1) during its catalytic cycle (2016)
Molecular Pharmacology, 90 (1), 35-41 - Cryo-EM of viruses and vaccine design (2016)
Proceedings of the National Academy of Sciences of the United States of America, 113 (32), 8903-8905 - Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating (2016)
Cell, 164 (4), 747-756 - CryoEM at IUCrJ: A new era (2016)
IUCrJ, 3, 3-7 - Imaging Cellular Architecture with 3D SEM (2016)
Encyclopedia of Cell Biology, 2, 44-50 - Mapping of ebolavirus neutralization by monoclonal antibodies in the ZMapp cocktail using cryo-electron tomography and studies of cellular entry (2016)
Journal of Virology, 90 (17), 7618-7627 - Resolution advances in cryo-EM enable application to drug discovery (2016)
Current Opinion in Structural Biology, 41, 194-202 - Structural basis for early-onset neurological disorders caused by mutations in human selenocysteine synthase (2016)
Scientific Reports, 6 - Structural basis of kainate subtype glutamate receptor desensitization (2016)
Nature, 537 (7621), 567-571 - The crystal structure of human GlnRS provides basis for the development of neurological disorders (2016)
Nucleic Acids Research, 44 (7), 3420-3431 - The democratization of cryo-EM (2016)
Nature Methods, 13 (8), 607-608 - Using cryo-EM to map small ligands on dynamic metabolic enzymes: Studies with glutamate dehydrogenase (2016)
Molecular Pharmacology, 89 (6), 645-651 - 2.2 å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor (2015)
Science, 348 (6239), 1147-1151 - A versatile nano display platform from bacterial spore coat proteins (2015)
Nature Communications, 6 - Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution (2015)
Nature Communications, 6 - Focused ion beams in biology (2015)
Nature Methods, 12 (11), 1021-1031 - Griffithsin tandemers: Flexible and potent lectin inhibitors of the human immunodeficiency virus (2015)
Retrovirology, 12 (1) - Maturation of the HIV-1 core by a non-diffusional phase transition (2015)
Nature Communications, 6 - Mitochondrial reticulum for cellular energy distribution in muscle (2015)
Nature, 523 (7562), 617-620 - A 3D cellular context for the macromolecular world (2014)
Nature Structural and Molecular Biology, 21 (10), 841-845 - Accelerating discovery in 3D microanalysis: Leveraging open source software and deskside high performance computing (2014)
Microscopy and Microanalysis, 20 (3), 774-775 - Multi-resolution correlative focused ion beam scanning electron microscopy: Applications to cell biology (2014)
Journal of Structural Biology, 185 (3), 278-284 - Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports (2014)
Scientific Reports, 4 - Single-particle cryo-electron microscopy (cryo-EM): Progress, challenges, and perspectives for further improvement (2014)
Advances in Imaging and Electron Physics, 185, 113-137 - Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography (2014)
Journal of Virology, 88 (18), 10958-10962 - Structural mechanism of glutamate receptor activation and desensitization (2014)
Nature, 514 (7522), 328-334 - Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy (2014)
Proceedings of the National Academy of Sciences of the United States of America, 111 (32), 11709-11714 - Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission (2014)
Journal of Virology, 88 (18), 10327-10339 - Three-dimensional imaging of viral infections (2014)
Annual Review of Virology, 1 (1), 453-473 - A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography (2013)
Journal of Structural Biology, 181 (2), 116-127 - Catching HIV 'in the act' with 3D electron microscopy (2013)
Trends in Microbiology, 21 (8), 397-404 - Complexes of neutralizing and non-neutralizing affinity matured fabs with a mimetic of the internal trimeric coiled-coil of HIV-1 gp41 (2013)
PLoS ONE, 8 (11) - Cryo-electron microscopy - A primer for the non-microscopist (2013)
FEBS Journal, 280 (1), 28-45 - Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation (2013)
PLoS ONE, 8 (11) - Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain (2013)
Proceedings of the National Academy of Sciences of the United States of America, 110 (15), 5921-5926 - HIV-1 envelope glycoprotein structure (2013)
Current Opinion in Structural Biology, 23 (2), 268-276 - HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1 (2013)
Journal of Virology, 87 (12), 7191-7196 - Molecular structures of trimeric HIV-1 Env in complex with small antibody derivatives (2013)
Proceedings of the National Academy of Sciences of the United States of America, 110 (2), 513-518 - Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy (2013)
Nature Structural and Molecular Biology, 20 (12), 1352-1357 - Protein Conformational Changes in the Bacteriorhodopsin Photocycle: Comparison of Findings from Electron and X-Ray Crystallographic Analyses (2013)
PLoS ONE, 4 (6) - Structure and accessibility of HA trimers on intact 2009 H1N1 pandemic influenza virus to stem region-specific neutralizing antibodies (2013)
Proceedings of the National Academy of Sciences of the United States of America, 110 (12), 4592-4597 - Structure of trimeric HIV-1 envelope glycoproteins (2013)
Proceedings of the National Academy of Sciences of the United States of America, 110 (45) - Targeted conformational search with map-restrained self-guided Langevin dynamics: Application to flexible fitting into electron microscopic density maps (2013)
Journal of Structural Biology, 183 (3), 429-440 - Chemical mapping of mammalian cells by atom probe tomography (2012)
Journal of Structural Biology, 178 (2), 98-107 - Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging (2012)
Journal of Structural Biology, 178 (2), 165-176 - Data management challenges in three-dimensional em (2012)
Nature Structural and Molecular Biology, 19 (12), 1203-1207 - Protein secondary structure determination by constrained single-particle cryo-electron tomography (2012)
Structure, 20 (12), 2003-2013 - Shape-based regularization of electron tomographic reconstruction (2012)
IEEE Transactions on Medical Imaging, 31 (12), 2241-2252 - Structural mechanism of trimeric HIV-1 envelope glycoprotein activation (2012)
PLoS Pathogens, 8 (7), 37 - There is no overkill in biochemistry: Har Gobind Khorana, a pioneer in membrane biology (2012)
Resonance, 17 (12), 1157-1164 - Visualizing cells and humans in 3D: Biomedical image analysis at nanometer and meter scales (2012)
IEEE Computer Graphics and Applications, 32 (5), 39-49 - Chemotaxis kinase CheA is activated by three neighbouring chemoreceptor dimers as effectively as by receptor clusters (2011)
Molecular Microbiology, 79 (3), 677-685 - Compositional mapping of the surface and interior of mammalian cells at submicrometer resolution (2011)
Analytical Chemistry, 83 (4), 1207-1213 - Correlative 3D imaging of whole mammalian cells with light and electron microscopy (2011)
Journal of Structural Biology, 176 (3), 268-278 - Determination of molecular structures of HIV envelope glycoproteins using cryo-electron tomography and automated sub-tomogram averaging (2011)
Journal of Visualized Experiments (58) - HIV-1 activates Cdc42 and induces membrane extensions in immature dendritic cells to facilitate cell-to-cell virus propagation (2011)
Blood, 118 (18), 4841-4852 - Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response (2011)
EMBO Journal, 30 (9), 1719-1729 - Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus (2011)
Journal of Bacteriology, 193 (6), 1341-1350 - Structural plasticity of a transmembrane peptide allows self-assembly into biologically active nanoparticles (2011)
Proceedings of the National Academy of Sciences of the United States of America, 108 (24), 9798-9803 - Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography (2011)
Journal of Virology, 85 (23), 12114-12123 - Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures (2011)
Proceedings of the National Academy of Sciences of the United States of America, 108 (28), 11440-11445 - 3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells (2010)
Proceedings of the National Academy of Sciences of the United States of America, 107 (30), 13336-13341 - A coiled-coil-repeat protein 'Ccrp' in Bdellovibrio bacteriovorus prevents cellular indentation, but is not essential for vibroid cell morphology (2010)
FEMS Microbiology Letters, 313 (2), 89-95 - Ion-abrasion scanning electron microscopy reveals distorted liver mitochondrial morphology in murine methylmalonic acidemia (2010)
Journal of Structural Biology, 171 (2), 125-132 - Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: Strain- dependent variation in quaternary structure (2010)
PLoS Pathogens, 6 (12) - 3D imaging of diatoms with ion-abrasion scanning electron microscopy (2009)
Journal of Structural Biology, 166 (3), 316-328 - 3D Imaging of mammalian cells with ion-abrasion scanning electron microscopy (2009)
Journal of Structural Biology, 166 (1), 1-7 - Automatic joint classification and segmentation of whole cell 3D images (2009)
Pattern Recognition, 42 (6), 1067-1079 - Cryo-electron tomography of bacteria: Progress, challenges and future prospects (2009)
Nature Reviews Microbiology, 7 (9), 666-675 - Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages (2009)
PLoS Pathogens, 5 (9) - Membrane protein structure determination using cryo-electron tomography and 3D image averaging (2009)
Current Opinion in Structural Biology, 19 (4), 402-407 - Staphylococcal enterotoxin A induces small clusters of HLA-DR1 on B cells (2009)
PLoS ONE, 4 (7) - Structural snapshots of conformational changes in a seven-helix membrane protein: lessons from bacteriorhodopsin (2009)
Current Opinion in Structural Biology, 19 (4), 433-439 - 3D imaging of diatoms with ion abrasion scanning electron microscopy (2008)
Microscopy and Microanalysis, 14 (SUPPL. 2), 972-973 - Chemoreceptors in Caulobacter crescentus: Trimers of receptor dimers in a partially ordered hexagonally packed array (2008)
Journal of Bacteriology, 190 (20), 6805-6810 - Classification and 3D averaging with missing wedge correction in biological electron tomography (2008)
Journal of Structural Biology, 162 (3), 436-450 - Electron tomography in nanoparticle imaging and analysis (2008)
Nanomedicine, 3 (1), 125-131 - Evaluation of denoising algorithms for biological electron tomography (2008)
Journal of Structural Biology, 164 (1), 7-17 - Extended Polypeptide Linkers Establish the Spatial Architecture of a Pyruvate Dehydrogenase Multienzyme Complex (2008)
Structure, 16 (1), 93-103 - Molecular Architecture of Native HIV-1 gp 120 Trimers (2008)
Chemtracts, 21 (6), 227-228 - Molecular architecture of native HIV-1 gp120 trimers (2008)
Nature, 455 (7209), 109-113 - Role of HAMP domains in chemotaxis signaling by bacterial chemoreceptors (2008)
Proceedings of the National Academy of Sciences of the United States of America, 105 (43), 16555-16560 - Stoichiometry and absolute quantification of proteins with mass spectrometry using fluorescent and isotope-labeled concatenated peptide standards (2008)
Molecular and Cellular Proteomics, 7 (2), 442-447 - Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography (2008)
Journal of Bacteriology, 190 (7), 2588-2596 - Automated 100-position specimen loader and image acquisition system for transmission electron microscopy (2007)
Journal of Structural Biology, 158 (3), 318-326 - Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography (2007)
Journal of Bacteriology, 189 (20), 7503-7506 - Classification, averaging and reconstruction of macromolecules in electron tomography (2007)
2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings, 244-247 - Cryoelectron tomographic analysis of an HIV-neutralizing protein and its complex with native viral gp120 (2007)
Journal of Biological Chemistry, 282 (38), 27754-27759 - Determination of protein structures in situ: Electron tomography of intact viruses and cells (2007)
2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings, 233-235 - Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy (2007)
Proceedings of the National Academy of Sciences of the United States of America, 104 (10), 3777-3781 - Electron Tomography of Bacterial Chemotaxis Receptor Assemblies (2007)
Methods in Cell Biology, 2007 (79), 373-384 - Electron tomography of the contact between T cells and SIV/HIV-1: Implications for viral entry (2007)
PLoS Pathogens, 3 (5), 0571-0581 - Electron tomography of viruses (2007)
Current Opinion in Structural Biology, 17 (5), 596-602 - From gigabytes to bytes: Automated denoising and feature identification in electron tomograms of intact bacterial cells (2007)
2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings, 304-307 - Gigabytes to bytes: Automated denoising and feature extraction as applied to the analysis of HIV architecture and variability using electron tomography (2007)
ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings, 1 - Regularization for inverting the radon transform with wedge consideration (2007)
2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings, 217-220 - Automated, 100-specimen loading and imaging system for transmission electron microscopy (2006)
Microscopy and Microanalysis, 12 (SUPPL. 2), 1100-1101 - Bridging the imaging gap in nanobiology with three-dimensional electron microscopy (2006)
Microscopy and Microanalysis, 12 (SUPPL. 2), 526-527 - Bridging the imaging gap in nanobiology with three-dimensional electron microscopy (2006)
2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems, 37 - Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy (2006)
Journal of Biological Chemistry, 281 (7), 4364-4370 - Site-specific 3D imaging of cells and tissues with a dual beam microscope (2006)
Journal of Structural Biology, 155 (1), 63-73 - Structure and function of icosahedral pyruvate dehydrogenase complexes (2006)
Microscopy and Microanalysis, 12 (SUPPL. 2), 26-27 - The SIV surface spike imaged by electron tomography: One leg or three? (2006)
PLoS Pathogens, 2 (8), 0727-0730 - An energy-based three-dimensional segmentation approach for the quantitative interpretation of electron tomograms (2005)
IEEE Transactions on Image Processing, 14 (9), 1314-1323 - Bridging the imaging gap: Visualizing subcellular architecture with electron tomography (2005)
Current Opinion in Microbiology, 8 (3), 316-322 - Electron tomography of degenerating neurons in mice with abnormal regulation of iron metabolism (2005)
Journal of Structural Biology, 150 (2), 144-153 - A new approach for 3D segmentation of cellular tomograms obtained using three-dimensional electron microscopy (2004)
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, 1, 5-8 - A template-propagation method for segmentation of filamentous structures in electron tomograms (2004)
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, 1, 1-4 - Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr (2004)
Journal of Microscopy, 216 (1), 76-83 - Site-specific 3D imaging of cells and tissues using DualBeam technology (2004)
Microscopy and Microanalysis, 10 (SUPPL. 2), 1124-1125 - Structure and transport mechanism of the bacterial oxalate transporter OxIT (2004)
Biophysical Journal, 87 (5), 3600-3607 - Three-dimensional electron microscopic imaging of membrane invaginations in Escherichia coli overproducing the chemotaxis receptor Tsr (2004)
Journal of Bacteriology, 186 (15), 5052-5061 - Three-dimensional electron microscopy at molecular resolution (2004)
Annual Review of Biophysics and Biomolecular Structure, 33, 141-155 - 3D imaging of membrane networks in a bacterial cell using electron tomography (2003)
Microscopy and Microanalysis, 9 (SUPPL. 2), 1168-1169 - A core-weighted fitting method for docking atomic structures into low-resolution maps: Application to cryo-electron microscopy (2003)
Journal of Structural Biology, 141 (1), 63-76 - Automated image acquisition and processing using a new generation of 4K × 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies (2003)
Journal of Structural Biology, 143 (2), 135-144 - Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr (2003)
Journal of Bacteriology, 185 (12), 3636-3643 - Projection structure of the bacterial oxalate transporter OxlT at 3.4 Å resolution (2003)
Journal of Structural Biology, 144 (3), 320-326 - Structural insights into the mechanism of proton pumping by bacteriorhodopsin (2003)
FEBS Letters, 545 (1), 2-8 - Structural model for 12-helix transporters belonging to the major facilitator superfamily (2003)
Journal of Bacteriology, 185 (5), 1712-1718 - From structure to mechanism: Electron crystallographic studies of bacteriorhodopsin (2002)
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 360 (1794), 859-874 - Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: A multifunctional catalytic machine (2002)
EMBO Journal, 21 (21), 5587-5598 - Molecular structure of an icosahedral pyruvate dehydrogenase complex (2002)
Microscopy and Microanalysis, 8 (SUPPL. 2), 214-215 - Proton translocation by bacteriorhodopsin in the absence of substantial conformational changes (2002)
Journal of Molecular Biology, 319 (2), 555-565 - Three-dimensional structure of a bacterial oxalate transporter (2002)
Nature Structural Biology, 9 (8), 597-600 - Automated data collection with a Tecnai 12 electron microscope: Applications for molecular imaging by cryomicroscopy (2001)
Journal of Structural Biology, 135 (3), 251-261 - Projection structure and molecular architecture of OxlT, a bacterial membrane transporter (2001)
EMBO Journal, 20 (16), 4408-4413 - CCD detectors in high-resolution biological electron microscopy (2000)
Quarterly Reviews of Biophysics, 33 (1), 1-27 - Crystallographic analysis of protein conformational changes in the bacteriorhodopsin photocycle (2000)
Biochimica et Biophysica Acta - Bioenergetics, 1460 (1), 157-165 - Integration of deletion mutants of bovine rhodopsin into the membrane of the endoplasmic reticulum (2000)
Molecular Membrane Biology, 17 (3), 165-174 - Cooled CCD detector with tapered fibre optics for recording electron diffraction patterns (1999)
Ultramicroscopy, 75 (4), 235-250 - Electron crystallography of bacteriorhodopsin with millisecond time resolution (1999)
Journal of Structural Biology, 128 (1), 19-25 - Protein conformational changes in the bacteriorhodopsin photocycle (1999)
Journal of Molecular Biology, 287 (1), 145-161 - The structure of bacteriorhodopsin: An emerging consensus (1999)
Current Opinion in Structural Biology, 9 (4), 462-468 - Electron diffraction studies of light-induced conformational changes in the Leu-93 → Ala bacteriorhodopsin mutant (1997)
Proceedings of the National Academy of Sciences of the United States of America, 94 (5), 1767-1772 - Evidence for a long-lived 13-cis-containing intermediate in the photocycle of the Leu 93 → Ala bacteriorhodopsin mutant (1997)
Journal of Physical Chemistry B, 101 (29) - Evidence for a long-lived 13-cis-containing intermediate in the photocycle of the Leu 93→Ala bacteriorhodopsin mutant (1997)
Journal of Physical Chemistry B, 101 (29), 5619-5621 - Expression of bacteriorhodopsin in Sf9 and COS-1 cells (1997)
Journal of Bioenergetics and Biomembranes, 29 (1), 55-59 - Expression, stability, and membrane integration of truncation mutants of bovine rhodopsin (1997)
Proceedings of the National Academy of Sciences of the United States of America, 94 (10), 4966-4971 - Reducing the flexibility of retinal restores a wild-type-like photocycle in bacteriorhodopsin mutants defective in protein-retinal coupling (1997)
Proceedings of the National Academy of Sciences of the United States of America, 94 (10), 5028-5033 - Structural changes underlying proton pumping by Bacteriorhodopsin (1997)
FASEB Journal, 11 (9) - Studies of rh1 metarhodopsin stabilization in wild-type drosophila and in mutants lacking one or both arrestins (1997)
Biochemistry, 36 (8), 2188-2196 - Modulation of arrestin release in the light-driven regeneration of Rh1 Drosophila rhodopsin (1996)
Biochemistry, 35 (6), 1848-1855 - The residues Leu 93 and Asp 96 act independently in the bacteriorhodopsin photocycle: Studies with the Leu 93→Ala, Asp 96→Asn double mutant (1996)
Biophysical Journal, 70 (5), 2366-2372 - Molecular mechanism of protein-retinal coupling in bacteriorhodopsin (1995)
Proceedings of the National Academy of Sciences of the United States of America, 92 (24), 11120-11124 - Activation and regeneration of rhodopsin in the insect visual cycle (1994)
Science, 266 (5189), 1369-1373 - Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin (1993)
EMBO Journal, 12 (1), 1-8 - Hydrophobic amino acids in the retinal-binding pocket of bacteriorhodopsin (1993)
Journal of Biological Chemistry, 268 (27), 20305-20311 - Aspartic acid 85 in bacteriorhodopsin functions both as proton acceptor and negative counterion to the schiff base (1992)
Journal of Biological Chemistry, 267 (36), 25730-25733 - Effect of introducing different carboxylate-containing side chains at position 85 on chromophore formation and proton transport in bacteriorhodopsin (1992)
Journal of Biological Chemistry, 267 (36), 25734-25738 - Replacement of leucine-93 by alanine or threonine slows down the decay of the N and O intermediates in the photocycle of bacteriorhodopsin: Implications for proton uptake and 13-cis-retinal → all-trans-retinal reisomerization (1991)
Proceedings of the National Academy of Sciences of the United States of America, 88 (15), 6873-6877 - The reaction of hydroxylamine with bacteriorhodopsin studied with mutants that have altered photocycles: Selective reactivity of different photointermediates (1991)
Proceedings of the National Academy of Sciences of the United States of America, 88 (6), 2583-2587 - Protonation state of Asp (Glu)-85 regulates the purple-to-blue transition in bacteriorhodopsin mutants Arg-82 → Ala and Asp-85 → Glu: The blue form is inactive in proton translocation (1990)
Proceedings of the National Academy of Sciences of the United States of America, 87 (3), 1013-1017 - Ultraviolet-visible transient spectroscopy of bacteriorhodopsin mutants: Evidence for two forms of tyrosine-185 → phenylalanine (1990)
Journal of Biological Chemistry, 265 (28), 16978-16984 - Replacement of aspartic residues 85, 96, 115, or 212 affects the quantum yield and kinetics of proton release and uptake by bacteriorhodopsin (1989)
Proceedings of the National Academy of Sciences of the United States of America, 86 (2), 529-533 - Critical mixing in monolayer mixtures of phospholipid and cholesterol (1987)
Journal of Physical Chemistry, 91 (7), 1715-1718 - Mono- and bilayers of phospholipids at interfaces: Interlayer coupling and phase stability (1985)
Journal of Physical Chemistry, 89 (17), 3592-3595 - Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker (1985)
Science, 227 (4685), 415-417 - Lateral diffusion of specific antibodies bound to lipid monolayers on alkylated substrates.
Proceedings of the National Academy of Sciences of the United States of America, 83
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