Kevin Bennewith

Associate Professor

Relevant Degree Programs

 

Graduate Student Supervision

Doctoral Student Supervision (Jan 2008 - May 2019)
Recruitment, phenotype and function of tumour-infiltrating regulatory T cells in the lung microenvironment (2018)

Despite the crucial role of regulatory T cells (Tregs) in the regulation of immunity toward innocuous antigens, these cells may contribute to the growth and metastasis of a variety of malignancies, either directly by production of cell growth and angiogenic factors, or indirectly by suppression of anti-tumour immunity. Tregs may support the development of both primary and metastatic tumours; however, the therapeutic inhibition of these cells is complicated by the presence of tissue-specific phenotypes and functions, which require further characterization based on the type of cancer and local microenvironment. We have observed that Treg accumulation in primary and metastatic tumours in the lung microenvironment is significantly increased in a clinical and pre-clinical setting, respectively. Therefore, in this thesis we hypothesize that Tregs promote tumour growth in the lungs. To explore this hypothesis, we examine the phenotype and function of tumour-infiltrating Tregs and inhibit their homing to observe the impact on tumour growth. Herein, we report that Tregs isolated from mice bearing metastatic tumours are functionally immune suppressive and enriched for expression of the homing receptor C-C chemokine receptor type 5 (CCR5). Inhibition of Tregs with the CCR5 antagonist, Maraviroc, reduced CCR5⁺ Treg accumulation and tumour burden in the lungs. We identify that Tregs accumulate during early stage disease in lung cancer patients, and show that oncogene-induced chemokine production promotes Treg migration. Further, we identify that oncogene-induced Treg migration is significantly reduced by antibody-mediated neutralization of the CCR5 ligand, CCL5 ex vivo. Finally, we report that a subset of Tregs expressing the interleukin 1 family receptor, ST2, may directly promote tumour progression by production of the growth factor amphiregulin (AREG) in response to the cognate ligand, IL-33 in the lung microenvironment. Taken together, these results provide evidence that Tregs are important contributors to tumour development in the lung microenvironment and identify signaling axes required for their recruitment and function. Our findings highlight the viability of homing inhibitors as an alternative mechanism to systemically ablating Tregs for the treatment of lung metastases.

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Master's Student Supervision (2010 - 2018)
Clinically relevant conventional dose of ionizing radiation enhances tumour cell migration in human breast cancer cell lines (2017)

It is estimated that >90% of cancer-related deaths are associated with the development and growth of tumour metastases. While tumour cell migration can be enhanced by high doses of ionizing radiation (IR) in vitro, the effect of lower, clinically relevant conventional IR doses on tumour cell migration and metastasis is unclear. I hypothesize that tumour cells that survive radiation therapy have a higher propensity to migrate in vitro and extravasate into the lungs in vivo, independent from radiation-induced changes in the solid tumour microenvironment. Breast cancer cell lines treated with 2.3Gy IR were imaged in real-time over 72h to quantify changes in single cell migration. EMT statuses of cell lines were determined using Western blot and flow cytometry. We used conditioned medium from irradiated cells to determine whether cellular migration was influenced by secreted factors. TGF-β ELISAs were used to elucidate its role in enhancing cell migration after IR. Pre-irradiated and sham treated breast tumour cells were IV-injected into mice to examine changes in lung extravasation. The mesenchymal MDA-MB-231 and LM2-4 cell lines treated with 2.3Gy of IR migrated a greater total distance and/or displaced further from the point of origin compared to untreated cells. No induction of EMT by 2.3Gy irradiation was observed, although MCF-7 cells migrated further from the point of origin after IR. Conditioned media from 2.3Gy treated tumour cells enhanced migration and displacement of untreated tumour cells. TGF-β ELISA analysis of supernatants from sham and 2.3Gy treated MDA-MB-231 cells revealed an almost two-fold increase in TGF-β1 72h post treatment. Chemokine antibody arrays revealed a number of up-regulated proteins after 2.3Gy treatment. 8 hours after IV injection, 2.3Gy pre-irradiated tumour cells was observed with enhanced lung colonization compared to sham controls. IR dose of 2.3Gy are sufficient to enhance migration of both non-metastatic and metastatic breast cancer cell lines independent of EMT. By quantifying changes in the metastatic ability of tumour cells treated with a clinically relevant dose of radiation, my findings will help to determine whether there is a need for additional administration of targeted secondary therapy to minimize tumour cell dissemination.

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Investigation into the role of eosinophils in pulmonary metastasis and primary tumours in mouse models of breast cancer (2017)

Eosinophils are multifunctional granulocytes with potent immune modulatory and cytotoxic capabilities. Despite the presence of eosinophils in various solid tumours and eosinophilia being a prognosistic indicator in some cancers, the role of this innate immune cell has been largely overlooked in the context of cancer. Specifically, the role of eosinophils in pulmonary metastasis is poorly described despite their prevalence in the lung and association with various pulmonary diseases. I sought to delineate the role of eosinophils in murine models of metastatic breast cancer using novel mouse models of genetic eosinophil deficiency (ddGATA) and by immunodepletion in orthotopic mouse models of cancer using an anti-IL-5 antibody. I hypothesize that eosinophils enhance pulmonary metastasis in mouse models of breast cancer, but do not affect the vascularization of the primary tumour. Eosinophils are increased 5.8-fold in the lungs of mice bearing 4T1 metastatic mammary tumours (p
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Effect of hypoxia-inducible secreted protein, tenascin c, on 4T1 tumour cells in vitro and in vivo (2014)

Introduction: Metastatic cancer is responsible for 90% of cancer related deaths. Past research focused mainly on the primary tumour, leaving the process of metastasis poorly understood. Poorly oxygenated (hypoxic) tumour cells express hypoxia inducible proteins and have a more aggressive and invasive phenotype correlated with poorer prognosis. Hypoxic tumour cells are responsible for increased angiogenesis, invasion, matrix deposition and remodelling along with many other functions.We hypothesize hypoxic tumour secreted proteins are responsible for promoting metastasis. Our aim is to identify these hypoxia inducible secreted proteins and determine mechanisms promoting metastasis. Methods: Mammary carcinoma cells (4T1 – metastatic and 67NR – non-metastatic) were placed in 1% O₂ (hypoxic) or 21% O₂ (normoxic) for 24 hours. Stable Isotope Labelling of Amino acids in Cell culture (SILAC) and mass spectrometry were used to perform a quantitative proteomic screen of conditioned medium. Proteins in 4T1 conditioned media, up-regulated in hypoxia and absent from the 67NR results represented candidate secreted proteins in metastasis. Tenascin C (TNC), a candidate protein identified from the proteomic screen was stably knocked down and over-expressed. In vitro, the Boyden chamber and wound healing assay were used to study invasion and migration. In vivo, metastasis was assessed using flow cytometry-based quantification of metastasized tumour cells in the murine lungs.Results: (TNC) was identified as a secreted protein with a role promoting metastasis in vivo through enhanced migratory ability. In vitro, knockdown of TNC in 4T1 enhanced migratory ability whereas over-expression decreased migratory ability. These results were contradictory to the expected results based on the hypothesized in vivo role. However, in vivo knockdown of TNC in 4T1 tumour cells resulted in a significant decrease in lung metastases. These results are consistent with the expected role of TNC in vivo. Conclusions: Despite the contradictory results in vitro, TNC had a positive metastatic role potentially through a migratory mechanism. TNC represents a potential new therapeutic drug target. Given the 4T1 cell line results, these data support further examination of the migratory role of TNC and how it promotes metastasis. In addition, TNC expression in other tumour cell lines including human breast cancer should be examined.

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Myeloid-derived suppressor cell accumulation in secondary target organs promotes metastatic growth in breast cancer (2014)

A role for bone marrow-derived cells (BMDCs) in promoting metastatic tumour growth is emerging. Previous work has shown accumulation of CD11b⁺ BMDCs in pre-metastatic niches in the lungs of mice bearing metastatic breast tumours, although questions remain about the precise identity of these cells and their potential long-term influence on metastatic growth. We studied the induction, identity, longevity, and function of CD11b⁺ BMDCs in tissues of mice bearing murine mammary tumours. Metastatic, but not non-metastatic, mammary tumours induced the accumulation of CD11b⁺Gr1⁺ cells, which we functionally identified as immunosuppressive myeloid-derived suppressor cells (MDSCs) using ex vivo assays. Unlike BMDCs associated with pre-metastatic niches in other breast tumour models, MDSCs were induced systemically, with levels increasing in metastatic target organs (lung, liver, bone-marrow) and in tissues that do not harbor metastases from these tumours (kidney, spleen). We also found that circulating MDSC levels can be used as a surrogate marker for monitoring MDSC accumulation in tissues. Primary tumour resection caused decreased serum levels of granulocyte-colony stimulating factor and MDSCs, but functional MDSCs remained elevated in the lungs for several weeks after tumour resection. These MDSCs were associated with enhanced subsequent pulmonary metastatic growth, providing evidence that MDSC induction by metastatic primary tumours helps create a long-lasting metastasis-promoting environment in lung tissue. In addition to surgery, we utilized gemcitabine (GEM), 5-fluorouracil (5-FU) and tirapazamine (TPZ) to target MDSCs in the lungs and spleen of tumour bearing mice. We administered GEM to mice with resected tumours in order to target residual MDSCs. Our data demonstrates that GEM significantly eliminates residual MDSC levels in the lungs which resulted in a decreased ability of 4T1 tumour cells to colonize the metastatic target organ. Taken together, our findings suggest that metastatic murine mammary carcinomas induce systemic elevation of MDSCs and highlight the potential importance of identifying patients with elevated MDSC levels. Additionally, our research provides support for therapeutic targeting of MDSCs in patients at risk of developing or re-developing metastatic disease.

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The Effect of Heme Oxygenase-1 Breast Cancer Metastasis In Vivo and In Vitro (2014)

No abstract available.

 
 

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