Xiaoyan Jiang
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The overall goal of my research program is to understand the molecular mechanisms and cellular functions of specific oncogenes, tumor suppressor genes, miRNAs and their target genes in the regulation of the properties of cancer/leukemic stem cells, signal transduction events, initiation and progression of human leukemia and drug resistance. The ultimate objective is to identify molecules and pathways that will lead to new, rationally designed, more effective, and less toxic, personalized molecularly targeted therapies. In particular, we are extremely interested in developing mechanism-based combination therapeutic strategies that can directly target drug-insensitive leukemic stem cells.
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Graduate Student Supervision
Doctoral Student Supervision (Jan 2008 - Nov 2020)
Tyrosine kinase inhibitor (TKI) therapies have been introduced into clinical practice with remarkable effects on chronic myeloid leukemia (CML). However, early relapse, acquired drug resistance and persistence of leukemic stem cells (LSCs) remain problematic. Improved treatments specifically targeting key molecular elements active in CML LSCs are needed. One candidate is the oncoprotein AHI-1 (Abelson helper integration site-1), which is highly deregulated in LSCs. It harbors two key domains, SH3 and WD40-repeat, which are known important mediators of protein-protein interactions. An AHI-1-mediated protein complex containing BCR-ABL and JAK2 has been shown to modulate transforming activity and TKI-response/resistance of CML LSCs. In this study, I investigated the functional roles of the AHI-1 SH3 domain in regulation of cellular resistance of primitive CML cells to TKIs. I showed that deletion of the SH3 domain of Ahi-1 significantly enhanced apoptotic response of BCR-ABL⁺ cells to TKIs compared to cells expressing full-length Ahi-1. I solved the crystal structure of the AHI-1 SH3 domain and identified several unique features, providing potential target sites for designing specific drugs. Using immunoprecipitation/mass spectrometry, I identified a novel protein interaction between AHI-1 and Dynamin-2 (DNM2), a GTPase, through the AHI-1 SH3 domain. I showed that DNM2 expression was significantly upregulated in CML stem/progenitor cells compared to normal bone marrow cells. I also determined that the AHI-1 SH3 domain and the proline rich domain of DNM2 were mainly responsible for their interaction. Most importantly, I identified a novel protein complex in CML cells, containing BCR-ABL, AHI-1 and DNM2. Furthermore, I demonstrated an oncogenic role of DNM2 in primitive CML cells by showing that knockdown of DNM2 greatly impaired the survival of CML stem/progenitor cells and sensitized them to TKI treatments. Lastly, I illustrated that DNM2 might be involved in deregulation of endocytosis, ROS production and autophagy in TKI-insensitive CML stem/progenitor cells. This study detailed the identification and characterization of the newly-identified BCR-ABL-AHI-1-DNM2 protein complex and described the oncogenic functions of DNM2 in primitive CML cells. It further suggested that targeting DNM2 may facilitate eradication of LSCs as a new treatment option in CML.
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The identification of BCR-ABL1 as the key molecular event in chronic myeloid leukemia (CML) has revolutionized treatment opportunities for early phase patients. Imatinib mesylate (IM) and other ABL1 tyrosine kinase inhibitors (TKIs) have been introduced into the clinic with remarkable effects. However, initial and acquired resistance, relapse and in particular, the persistence of CML stem cells upon TKI therapy represent critical challenges and warrant the identification of predictive biomarkers and novel, distinct targets for improved treatment strategies. In this work, I investigated how CML stem and progenitor cells survive TKI therapy through intrinsic and bone marrow (BM) niche-associated mechanisms. I revealed that the core autophagy protease ATG4B, and the focal adhesion protein and serine/threonine kinase Integrin-linked kinase (ILK) play crucial roles in CML, and that they can be successfully targeted with small molecule inhibitors. By comparing the expression of various core autophagy genes and proteins, ATG4B was identified as potential biomarker in CML to predict IM-responders versus IM-nonresponders prior to the initiation of therapy. Furthermore, my studies illustrated that deregulation of ATG4B is critical to autophagy, survival and growth of CML stem and progenitor cells. Inhibition or suppression of ATG4B decreased CML cell viability significantly and sensitized leukemic cells to TKI treatment highlighting ATG4B as a novel target in CML. ILK was identified as a differentially expressed gene between CD34⁺ CML patient cells and healthy donors by RNA-sequencing (RNA-seq) analysis, and the importance of the ILK protein and its kinase functions in mediating TKI responses and resistance in CML stem and progenitor cells was demonstrated by ILK inhibitor (QLT0267) and ILK suppression studies. Moreover, various in vitro and in vivo assays showed that the simultaneous kinase inhibition of ILK and BCR-ABL1 is effective in targeting both leukemic stem and progenitor cells, including quiescent CML cells, and in the presence of stromal cells of the BM microenvironment that make TKI monotherapies ineffective. Overall, these studies provide the first evidence of the importance of ATG4B and ILK in CML, and their potential as novel therapeutic targets for improved combination treatments with TKIs to specifically eliminate CML stem and progenitor cells.
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Cutaneous T-cell lymphoma (CTCL) is a group of lymphoproliferative disorders consisting of two main subtypes: mycosis fungoides (MF) and Sézary syndrome (SS). Due to the lack of robust histological markers, it remains a challenge to establish an accurate diagnosis and offer long term prognostication for CTCL. In addition, the molecular pathogenesis of CTCL is only partially understood. Previously our group discovered that early stage MF skin biopsies contained ectopic expression of TOX gene, which is essential for the early development of CD4⁺ T cells but normally is switched off in mature CD4⁺ T cells in the peripheral tissues. The objectives of my thesis research are to evaluate if TOX can be used to improve CTCL diagnosis and prognostication, and to characterize the functional role of TOX in the pathogenesis of CTCL.Using skin biopsies and clinical databases from Vancouver, Beijing and Boston, I confirmed that TOX expression levels were significantly upregulated in the full spectrum of MF and in SS. In addition, as a diagnostic marker, high TOX expression levels differentiated CTCL from non-CTCL controls with good sensitivity and specificity. Furthermore, as a prognostic marker, high TOX mRNA levels correlated with increased risks of disease progression and disease-specific mortality in MF, and increased risks of disease-specific mortality in SS.I also investigated the functional role of TOX in CTCL pathogenesis using multiple CTCL cell lines and a mouse xenograft model. TOX knockdown in three CTCL cell lines led to markedly increased apoptosis, reduced cell proliferation, and impaired tumorigenic ability. These effects were partially mediated by increased expression of two cell cycle regulators, CDKN1B and CDKN1C. In addition, transcriptome analysis between TOX-suppressed cells and control CTCL cells uncovered additional potential molecules downstream of TOX, such as tumor suppressors FOXO3 and HBP1.Our results provide strong evidence that aberrant activation of TOX can serve as a diagnostic and prognostic biomarker for CTCL. Further, we demonstrated that TOX plays a crucial oncogenic role in CTCL pathogenesis, partially through regulating transcription of CDKN1B, CDKN1C and other downstream genes. Therefore TOX and/or its downstream genes may be promising therapeutic targets for CTCL.
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Chronic myeloid leukemia (CML) has long served as a paradigm for new insights into the cellular origin, pathogenesis and treatment of human cancers. ABL tyrosine kinase inhibitor (TKI) therapies have had remarkable effects on treatment of early phase CML. However, TKI monotherapies are not curative, and initial and acquired TKI resistance remain clinically challenging. Particularly, CML stem/progenitor cells are insensitive to TKIs. Therefore, novel treatments and predictive biomarkers are clearly needed. In this work, I studied the biological effects of dual BCR-ABL and JAK2 suppressions on TKI-nonresponder stem/progenitor cells, and identified and characterized novel microRNA (miRNA) biomarkers in these cells. I examined the biological effects of a new JAK2 inhibitor, BMS-911543, in combination with TKIs on CD34⁺ CML cells from IM-nonresponders. I demonstrated that combination therapy significantly reduces JAK2/STAT5 and CRKL activities, induces apoptosis, inhibits colony growth, and eliminates leukemic stem cells in vitro, while sparing healthy counterparts. I further showed that oral BMS-911543 combined with dasatinib is more effective in eliminating leukemic cells in an aggressive mouse model of BCR-ABL⁺ human leukemia. Next, I identified differentially expressed miRNAs in CD34⁺ CML cells using RNA-seq analysis, and validated the results in additional samples using high-throughput qPCR. Potential miRNA target genes were also identified by integrating miRNA expression profiles with gene expression profiles using strand-specific RNA-seq. These studies revealed that expression of miR-185 is significantly reduced in CD34⁺ CML cells from TKI-nonresponders compared to TKI-responders. Restoration of miR-185 expression by lentiviral transduction in CD34⁺ TKI-nonresponder cells significantly impairs survival of these cells and sensitizes them to TKI treatment in vitro and in vivo. Additionally, I validated the target genes of miR-185 to rationalize its roles in CML. Lastly, I demonstrated that the expression levels of several miRNAs, including miR185, were restored in patients treated with nilotinib, suggesting their potential as biomarkers to predict clinical response to TKI therapies.These studies have uncovered the biological significance of JAK2 and miR-185 in regulation of the properties of drug-insensitive CML stem/progenitor cells, and their potential as therapeutic targets for improved treatments with TKIs especially in patients at risk of developing TKI resistance.
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Cutaneous T-cell lymphomas (CTCLs) represent a group of lymphoproliferative disorders characterized by homing of malignant T-cells to the skin’s surface. There are two main types of CTCL: Mycosis Fungoides (MF) and Sezary Syndrome (SS). We have demonstrated that expression of the Abelson helper integration site-1 (AHI-1) oncogene is significantly increased in CD4⁺CD7- cells from SS patients. Bridging integrator 1 (BIN1) has been identified by microarray analysis of CTCL cells as a candidate gene involved in AHI-1-mediated lymphomagenesis. Interestingly, BIN1expression is significantly reduced in SS patient samples. However, the role of BIN1 and its molecular connection to AHI-1 in lymphomagenesis remains unexplored. I extensively investigated the role of key BIN1 isoforms in primary and CTCL cell line model systems both in vitro and in vivo. I demonstrated that overexpression/restored expression of BIN1 isoforms has strong anti-proliferative and pro-apoptotic roles in CTCL cells in vitro, and significantly inhibits the tumorigenic activity of these cells in vivo. The pro-apoptotic role of BIN1 in CTCL cells occurs through downregulation of c-FLIP, a critical inhibitor of Fas/FasL-mediated apoptosis. I also observed significant reduction and increase in BIN1 and c-FLIP transcripts in primary CTCL samples, respectively. Interestingly, high BIN1 and low c-FLIP transcripts correlated with better survival rate in SS patients. Thus, BIN1 deficiency may play an important role in CTCL pathogenesis by causing apoptosis resistance.Furthermore, I explored potential mechanisms by which AHI-1 leads to downregulation of BIN1, by (1) examining if AHI-1 physically interacts with BIN1; and (2) determining if AHI-1 alters transcription of BIN1 by changing the methylation status of the BIN1 promoter. These experiments did not yield direct evidence of these two potential mechanisms of AHI-1’s role in BIN1 suppression. Thus, the mechanism by which AHI-1 regulates BIN1 remains unknown. Nevertheless, several potential BIN1 interacting proteins were uncovered in CTCL cells, including α/β-tubulin and β-actin. Overall, this study provides the first evidence of strong tumor suppressor activity of BIN1 in CTCL. It points to the loss of BIN1 and subsequent upregulation of c-FLIP as an important mechanism to induce apoptosis resistance in CTCL cells, and identifies BIN1 and c-FLIP as potential CTCL therapeutic targets.
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Master's Student Supervision (2010 - 2018)
Treatment of chronic myeloid leukemia (CML) targets the BCR-ABL1 fusion oncoprotein that characterizes its pathogenesis using tyrosine kinase inhibitors (TKIs); however, drug resistance and relapse can occur when BCR-ABL1-independant survival pathways such as autophagy are activated. Our lab found that the key autophagy enzyme ATG4B is upregulated in CML stem/progenitor cells from patients that clinically do not respond to TKIs vs. patients that do. Knockdown of ATG4B was found to suppress autophagy and sensitize CML cells to TKIs. This study investigates if combined suppression of BCR-ABL1 and ATG4B by novel ABL1 and ATG4B inhibitors in autophagy-inducing conditions may present a novel therapeutic approach to overcome TKI-resistance in CML. I found that inhibition of ATG4B by DB2 significantly inhibits growth and induces apoptosis in CML cell lines alone and in combination with TKIs when autophagy is induced during serum deprivation. There also is a decrease in colony forming cells after DB2+TKI treatment compared to TKIs alone in non-responding CML cells (p
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Chronic myeloid leukemia (CML) is a hematological malignancy characterized by the presence of a novel fusion oncoprotein called BCR-ABL1 in a hematopoietic stem cell. BCR-ABL1 has constitutively active tyrosine kinase activity and deregulates many intracellular signaling pathways contributing to cancer formation, maintenance, and progression. The consistent genetic aberration of BCR-ABL1 in CML led to the development of the first molecularly-targeted cancer therapy called imatinib (IM), which revolutionized the treatment of early phase CML. However, IM is not curative, and 40% of patients with advanced CML experience primary intolerance or acquire resistance to IM. In addition, leukemic stem cells (LSCs) are relatively insensitive to IM and do not exclusively rely on BCR-ABL1 for survival. Therefore, it is important to investigate alternative pathways that are critical for LSC maintenance, to develop a strategy to eradicate them. The Hedgehog (HH) pathway, and particularly the protein Smoothened (SMO), has recently been described to be essential for CML LSCs in a mouse model. I hypothesized that the HH pathway was critical for the survival of CML stem/progenitor cells, and that dual inhibition of BCR-ABL1 and SMO would be superior to either alone in killing CML LSCs. I used a variety of biological and molecular assays to investigate expression changes of several HH pathway-associated genes and the functionality of different leukemic cell subsets from primary CML patient samples. I observed that HH pathway genes SMO and GLI2 were upregulated in CML compared with healthy bone marrow controls, and were more highly expressed in CD34⁺ cells from IM non-responders as compared with responders. In addition, these genes were most highly expressed in the stem cell-enriched Lin-CD34⁺CD38ˉ subpopulation in IM non-responders compared with progenitors and mature leukemic cells in the same patients. I also observed that CD34⁺ IM non-responder cells were more sensitive to SMO inhibition compared with responders in terms of viability, apoptosis, re-plating potential, and colony-forming ability following long-term culture.Taken together, my results support the hypothesis that the HH pathway is more critical for primitive CML cells from IM non-responders, and may represent a mechanism by which drug-resistant cells evade eradication by TKIs.
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Imatinib Mesylate (IM) and other tyrosine kinase inhibitors (TKIs) have had a major impact on treatment of early phase Chronic Myeloid Leukemia (CML) patients. However, TKI monotherapies are not curative and initial and acquired resistance remain challenges. Particularly, CML stem cells are less responsive to TKIs and are a critical target population for TKI resistance. Thus, improved treatments targeting key elements active in CML stem cells are needed. One candidate is Abelson helper integration site-1 (AHI-1), an oncogene that is highly upregulated in CML stem cells and interacts with multiple kinases, including BCR-ABL and JAK2. AHI-1-mediated complexes regulate TKI response/resistance of CML stem/progenitor cells, indicating that AHI-1 is a new therapeutic target in CML. By screening the Prestwick Chemical Library, a specific growth inhibitory compound that potentially targets AHI-1 was identified: Cantharidin (CAN), an inhibitor of protein phosphatase 2A (PP2A). CAN is toxic however, so two new PP2A inhibitors, LB100 and LB102, were identified for this study. These new inhibitors specifically inhibit PP2A activity and suppress growth of CML cell lines. Importantly, these new PP2A inhibitors selectively target CML stem/progenitor cells while sparing healthy stem/progenitor cells. When combined with TKIs there is significant further suppression of growth in cell lines and in CD34+ treatment-naïve IM-nonresponder cells. Furthermore, this combination effect was determined to be synergistic. Cell cycle analysis showed that treatment with PP2A inhibitors alone induced a shift from G1 to G2/M phase. Confocal microscopy confirmed that the G2/M arrest led to mitotic catastrophe. However a similar shift in cell population was observed after combination with IM, suggesting that the G2/M phase arrest is solely due to PP2A inhibition. Mechanistically, the PP2A-PR55α subunit was identified as a new AHI-1 interacting protein. Western blot analysis showed that, compared to single agents, the combination treatment greatly suppresses protein expression of AHI-1, BCR-ABL, JAK2, STAT5, AKT, β-catenin, P-38 and JNK. The combination treatment also affected PP2A and BCR-ABL-mediated β-catenin dephosphorylation/phosphorylation. These results indicate that simultaneously targeting both BCR-ABL and PP2A activities in CML stem/progenitor cells may provide a novel treatment option for CML patients, through destabilization of the protein-protein interactions mediated by AHI-1.
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C-terminal tensin-like protein (Cten) is a focal adhesion protein with no or limited protein expression in normal tissues, which has recently been reported to be overexpressed and act as an oncoprotein in numerous cancers. Since its expression status in human cutaneous melanoma is currently unknown, I used tissue microarrays and immunohistochemical staining to examine the protein expression of Cten throughout melanoma progression. I found that Cten was significantly up-regulated in dysplastic nevi (DN) compared to normal nevi (NN), and in primary melanoma (PM) compared to both DN and NN. Strong Cten staining was associated with a poorer 5- and 10-year overall and disease-specific survival for PM patients, and was an adverse independent prognostic factor for the 5-year survival of the same patients. In vitro studies using two melanoma cell lines supported these findings and indicated that Cten functions as an oncogene in melanoma.Since relatively little is known about how Cten contributes to tumorigenesis, I next investigated the expression profile of the RhoGAP Deleted in Liver Cancer-1 (DLC1), the only protein known to bind to Cten, in melanomas. Both cytoplasmic and nuclear DLC1 were detected, and both were down-regulated in metastatic melanoma (MM) compared to PM and nevi, with nuclear DLC1 expression additionally being reduced in PM compared to nevi. Both cytoplasmic and nuclear DLC1 were associated with the 5-year overall and disease-specific survival of all melanoma and MM patients, and with the disease-specific 10-year survival of all melanoma patients. Combined analysis of cytoplasmic and nuclear DLC1 revealed that for MM patients, concurrent loss of both cytoplasmic and nuclear DLC1 was associated with the worst survival outcome, with loss of either or both forms being a significant adverse independent prognostic factor for the 5-year survival of all melanoma and MM patients. A preliminary investigation into the relationship between Cten and DLC1 indicated that the effects of Cten on patient survival were dependent on the levels of DLC1, as expected.In summary, I here provide an initial characterization of the expression status and role of Cten in melanomagenesis, and speculate that it functions partly via interactions with the tumour suppressor DLC1.
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Chronic myeloid leukemia is a myeloproliferative disorder characterized by the presence of the Philadelphia chromosome, encoding a unique fusion gene BCR-ABL. The current first line treatment for patients diagnosed with CML involves administration of the ABL kinase inhibitor imatinib mesylate (IM). However, early relapses and acquired drug resistance remain a current impediment to successful treatment for many patients. This suggests the necessity for alternate treatment options which may include combination therapy targeting multiple vital proteins involved in the malignancy of the leukemia. AHI-1 (Abelson helper integration site 1) is a recently discovered oncogene that is highly deregulated in murine lymphomas and leukemias. AHI-1 displays a significant pattern of overexpression in a Philadelphia chromosome positive cell line K562 cells. To investigate AHI-1’s involvement in CML, AHI-1 was either stably overexpressed or suppressed in K562 cells. Interestingly, an increase in cellular proliferation and colony formation and a decrease in apoptosis were observed in the presence of IM when AHI-1 was overexpressed, while suppression of AHI-1 had the opposite effects. Phosphorylation and total protein expression levels of several proteins known to be involved in BCR-ABL signalling were quantified. Interestingly, elevated phosphorylation and total gene/protein expression levels of several of these proteins were observed when AHI-1 was overexpresessed, in particular NF-κB and JAK2/STAT5 displayed increased expression. Due to the strong effects AHI-1 had on the JAK2/STAT5 signalling cascade, we then inhibited JAK2 activity using a new JAK2 inhibitor, TG101209. AHI-1 overexpression led to a reduction in the cellular response to the inhibitor while suppression of AHI-1 caused an increase in sensitivity in viability, apoptosis, and colony forming cell assays. Finally, a combination of IM and TG101209 was examined in the same K562 cells lines. Results from suggest that using a combination treatment approach was more effective at inhibiting cellular viability and colony formation than either treatment alone. These findings together suggest that AHI-1 may play an important role in mediating cellular resistance to IM and TG101209 and activates several BCR-ABL signalling pathways, and that it may be a vital target in eradicating the malignant leukemic cells arising in CML.
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Recent Tri-Agency Grants
The following is a selection of grants for which the faculty member was principal investigator or co-investigator. Currently, the list only covers Canadian Tri-Agency grants from years 2013/14-2016/17 and excludes grants from any other agencies.
- Identification of new microRNA biomarkers and candidate target genes in primitive CML cells using global comparative RNA analysis - Canadian Institutes of Health Research (CIHR) - Travel Awards - Institute Community Support (2015/2016)
- Targeting Drug-Insensitive CML Stem Cells with Combination Therapy of New ABL and PP2A Inhibitors - Canadian Institutes of Health Research (CIHR) - Operating Grant (2014/2015)
- Molecular Targeting of Critical Domains of AHI-1 Oncoprotein in Chronic Myeloid Leukemia Stem Cells Resistant to Current Tyrosine Kinase Inhibitor Therapy - Canadian Institutes of Health Research (CIHR) - Operating Grant (2013/2014)
- CCSRI #2010-700289 JIANG - Canadian Institutes of Health Research (CIHR) - (2013/2014)
- Abnormal activation of thymocytes high-mobility group box protein (TOX) in cutaneous T cell lymphomas: Pathogenic significance and clinical applications - Canadian Institutes of Health Research (CIHR) - Operating Grant (2013/2014)
Publications
- Cholesterol grafted cationic lipopolymers: Potential siRNA carriers for selective chronic myeloid leukemia therapy (2020)
Journal of Biomedical Materials Research - Part A, 108 (3), 565-580 - Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase Inhibitors (2020)
Cell Stem Cell, 27 (1), 110-124.e9 - Puncta intended: connecting the dots between autophagy and cell stress networks (2020)
Autophagy - The miR-185/PAK6 axis predicts therapy response and regulates survival of drug-resistant leukemic stem cells in CML (2020)
Blood, 136 (5), 596-609 - BCR-Abl silencing by siRNA: A potent approach to sensitize chronic myeloid leukemia cells to tyrosine kinase inhibitor therapy (2019)
Stem Cells and Development, 28 (11), 734-744 - Bone marrow–derived mesenchymal stromal cells promote resistance to tyrosine kinase inhibitors in chronic myeloid leukemia via the IL-7/JAK1/STAT5 pathway (2019)
Journal of Biological Chemistry, 294 (32), 12167-12179 - Current outlook on autophagy in human leukemia: Foe in cancer stem cells and drug resistance, friend in new therapeutic interventions (2019)
International Journal of Molecular Sciences, 20 (3) - Current outlook on drug resistance in chronic myeloid leukemia (CML) and potential therapeutic options (2019)
Drug Discovery Today, 24 (7), 1355-1369 - Diverse mechanisms of autophagy dysregulation and their therapeutic implications: does the shoe fit? (2019)
Autophagy, 15 (2), 368-371 - Implementing sirna-based therapy of chronic myeloid leukemia ex vivo by using lipopolymeric carriers (2019)
Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, 40, 542 - Response to Comment on "pP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL+ human leukemia" (2019)
Science Translational Medicine, 11 (501) - siRNA-mediated BCR-ABL silencing in primary chronic myeloid leukemia cells using lipopolymers (2019)
Journal of Controlled Release, 310, 141-154 - B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies (2018)
Cell, 173 (2), 470-484.e18 - Molecular mechanism of panaxydol on promoting axonal growth in PC12 cells (2018)
Neural Regeneration Research, 13 (11), 1927-1936 - PP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL+ human leukemia (2018)
Science Translational Medicine, 10 (427) - siRNA/lipopolymer nanoparticles to arrest growth of chronic myeloid leukemia cells in vitro and in vivo (2018)
European Journal of Pharmaceutics and Biopharmaceutics, 130, 66-70 - A novel AHI-1-BCR-ABL-DNM2 complex regulates leukemic properties of primitive CML cells through enhanced cellular endocytosis and ROS-mediated autophagy (2017)
Leukemia, 31 (11), 2376-2387 - CuboCube: Student creation of a cancer genetics e-textbook using open-access software for social learning (2017)
PLoS Biology, 15 (3) - Synergistic SNGI-TKI combination against LSCs (2017)
Aging, 9 (7), 1643-1644 - Targeting BCR-ABL+ stem/progenitor cells and BCR-ABL-T315I mutant cells by effective inhibition of the BCR-ABL-Tyr177-GRB2 complex (2017)
Oncotarget, 8 (27), 43662-43677 - Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2016)
Autophagy, 12 (1), 1-222 - New mouse models to investigate the efficacy of drug combinations in human chronic myeloid leukemia (2016)
Methods in Molecular Biology, 1465, 187-205 - BIN1 tumor suppressor regulates Fas/Fas ligand-mediated apoptosis through c-FLIP in cutaneous T-cell lymphoma (2015)
Leukemia, 29 (6), 1402-1413 - Evidence of an oncogenic role of aberrant TOX activation in cutaneous T-cell lymphoma (2015)
Blood, 125 (9), 1435-1443 - Precision autophagy: Will the next wave of selective autophagy markers and specific autophagy inhibitors feed clinical pipelines? (2015)
Autophagy, 11 (10), 1949-1952 - Progress in RNAi-mediated molecular therapy of acute and chronic myeloid leukemia (2015)
Molecular Therapy - Nucleic Acids, 4 (5), e240 - Distinguishing CML LSCs from HSCs using CD26 (2014)
Blood, 123 (25), 3851-3852 - Polymeric nanoparticle-mediated silencing of CD44 receptor in CD34+ acute myeloid leukemia cells (2014)
Leukemia Research, 38 (11), 1299-1308 - Selective JAK2/ABL dual inhibition therapy effectively eliminates TKI-insensitive CML stem/progenitor cells (2014)
Oncotarget, 5 (18), 8637-8650 - SiRNA therapy in cutaneous T-cell lymphoma cells using polymeric carriers (2014)
Biomaterials, 35 (34), 9382-9394 - The core autophagy protein ATG4B is a potential biomarker and therapeutic target in CML stem/progenitor cells (2014)
Blood, 123 (23), 3622-3634 - Thymocyte selection-associated high mobility group box gene (TOX) is aberrantly over-expressed in mycosis fungoides and correlates with poor prognosis (2014)
Oncotarget, 5 (12), 4418-4425 - TNFRSF11B polymorphisms are associated with metabolic traits in Uyghur and Han ethnic groups (2014)
Endocrine Research, 39 (1), 34-38 - 12(S)-hydroxyeicosatetraenoic acid levels link to coronary artery disease in Type 2 diabetic patients (2013)
Journal of Endocrinological Investigation, 36 (6), 385-389 - A strain of Siniperca chuatsi rhabdovirus causes high mortality among cultured largemouth bass in South China (2013)
Journal of Aquatic Animal Health, 25 (3), 197-204 - Autophagy: From structure to metabolism to therapeutic regulation (2013)
Autophagy, 9 (12), 2180-2182 - Genotypic and functional diversity of phenotypically defined primitive hematopoietic cells in patients with chronic myeloid leukemia (2013)
Experimental Hematology, 41 (10), 837-847 - Investigating siRNA delivery to chronic myeloid leukemia K562 cells with lipophilic polymers for therapeutic BCR-ABL down-regulation (2013)
Journal of Controlled Release, 172 (2), 495-503 - Long-term deregulated human hematopoiesis in goats transplanted in utero with BCR-ABL-transduced lin- CD34+ cord blood cells (2013)
Cell Research, 23 (6), 859-862 - PGC-1α prevents apoptosis in adipose-derived stem cells by reducing reactive oxygen species production in a diabetic microenvironment (2013)
Diabetes Research and Clinical Practice, 100 (3), 368-375 - Targeting primitive chronic myeloid leukemia cells by effective inhibition of a new AHI-1-BCR-ABL-JAK2 complex (2013)
Journal of the National Cancer Institute, 105 (6), 405-423 - Complete genomic sequence of a reovirus isolated from grass carp in China (2012)
Virus Research, 163 (1), 275-283 - CYP7A1 polymorphism influences the LDL cholesterol-lowering response to atorvastatin (2012)
Journal of Clinical Pharmacy and Therapeutics, 37 (6), 719-723 - Effective Non-Viral Delivery of siRNA to Acute Myeloid Leukemia Cells with Lipid-Substituted Polyethylenimines (2012)
PLoS ONE, 7 (8) - Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL+ chronic myeloid leukemia cells to tyrosine kinase inhibitors (2012)
Proteomics, 12 (13), 2094-2106 - Overexpression of SKP2 promotes the radiation resistance of esophageal squamous cell carcinoma (2012)
Radiation Research, 177 (1), 52-58 - Progress in stem cell therapy for the diabetic foot (2012)
Diabetes Research and Clinical Practice, 97 (1), 43-50 - AHI-1: A novel signaling protein and potential therapeutic target in human leukemia and brain disorders (2011)
Oncotarget, 2 (12), 918-934 - Characterization of a ranavirus isolated from cultured largemouth bass (Micropterus salmoides) in China (2011)
Aquaculture, 312 (1-4), 198-204 - Deficiency of SATB1 expression in Sézary cells causes apoptosis resistance by regulating FasL/CD95L transcription (2011)
Blood, 117 (14), 3826-3835 - Expression and function of miRNA in postoperative radiotherapy sensitive and resistant patients of non-small cell lung cancer (2011)
Lung Cancer, 72 (1), 92-99 - Identification and molecular typing of Streptococcus agalactiae isolated from pond-cultured tilapia in China (2011)
Fisheries Science, 77 (4), 623-632 - Improvement of production procedure for inactivated vaccine against Aeromonas hydrophila J-1 strain (2011)
Chinese Journal of Biologicals, 24 (3), 345-348 - The expression and function of miRNA-451 in non-small cell lung cancer (2011)
Cancer Letters, 311 (2), 203-209 - [The relationship between bone mass and the skin fold thickness in Chinese Hans]. (2010)
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 27 (4), 865-867 - A polymorphism near osteoprotegerin gene confer risk of obesity in Uyghurs (2010)
Endocrine, 37 (3), 383-388 - Analysis of BCR-ABL1 tyrosine kinase domain mutational spectra in primitive chronic myeloid leukemia cells suggests a unique mutator phenotype (2010)
Leukemia, 24 (10), 1817-1821 - Association of LPR5 polymorphism with bone mass density and cholesterol level in population of chinese han (2010)
Experimental and Clinical Endocrinology and Diabetes, 118 (6), 388-391 - Differential expression of connexin 43 in human autoimmune thyroid disease (2010)
Acta Histochemica, 112 (3), 278-283 - Expression of miRNA-130a in nonsmall cell lung cancer (2010)
American Journal of the Medical Sciences, 340 (5), 385-388 - Expression of the leukemic prognostic marker CD7 is linked to epigenetic modifications in chronic myeloid leukemia (2010)
Molecular Cancer, 9 - Identification of E74-like factor 1 (ELF1) as a transcriptional regulator of the Hox cofactor MEIS1 (2010)
Experimental Hematology, 38 (9), 798-808.e2 - Insights into the stem cells of chronic myeloid leukemia (2010)
Leukemia, 24 (11), 1823-1833 - Properties of CD34+ CML stem/progenitor cells that correlate with different clinical responses to imatinib mesylate (2010)
Blood, 116 (12), 2112-2121 - Association of ER-Alpha gene polymorphism with metabolic phenotypes in Chinese hans (2009)
Lipids, 44 (8), 719-723 - EGb761 protects hydrogen peroxide-induced death of spinal cord neurons through inhibition of intracellular ROS production and modulation of apoptotic regulating genes (2009)
Journal of Molecular Neuroscience, 38 (2), 103-113 - Expressions of connexin 43, 26 in thyroid epithelial cells from the patients with autoimmune thyroid diseases (2009)
Chinese Journal of Endemiology, 28 (6), 619-621 - Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells (2009)
Blood, 113 (19), 4646-4655 - AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells (2008)
Journal of Experimental Medicine, 205 (11), 2657-2671 - An approach to the management of chronic myeloid leukemia in British Columbia (2008)
Current Oncology, 15 (2), 48-55 - Correlation among bone density, bone mineral content and body mass index: An investigation of 287 Chinese Han volunteers (2008)
Journal of Clinical Rehabilitative Tissue Engineering Research, 12 (42), 8315-8318 - Huntingtin-associated protein 1 interacts with Ahi1 to regulate cerebellar and brainstem development in mice (2008)
Journal of Clinical Investigation, 118 (8), 2785-2795 - Neuroprotective effect of citicoline on dopaminergic neuron injury induced by MPP+ in mouse mesencephalic dissociated culture (2008)
Journal of Jilin University Medicine Edition, 34 (1), 53-56 - Panaxydol and panaxynol protect cultured cortical neurons against Aβ25-35-induced toxicity (2008)
Neuropharmacology, 54 (5), 845-853 - Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway (2008)
Neuroscience, 151 (1), 138-147 - Stem cell biomarkers in chronic myeloid leukemia (2008)
Disease Markers, 24 (4-5), 201-216 - Chronic myeloid leukemia stem cells possess multiple unique features of resistance to BCR-ABL targeted therapies (2007)
Leukemia, 21 (5), 926-935 - Effects of low dose radiation on activity of reactive oxygen species and mitochondrion membrane potential in spermatogenic cells of mouse testes (2007)
Journal of Jilin University Medicine Edition, 33 (5), 786-789 - Instability of BCR-ABL gene in primary and cultured chronic myeloid leukemia stem cells (2007)
Journal of the National Cancer Institute, 99 (9), 680-693 - The challenges of targeting chronic myeloid leukemia stem cells (2007)
Clinical Lymphoma and Myeloma, 7 (SUPPL. 2) - Toward distinguishing LSCs from HSCs (2007)
Blood, 110 (7), 2222-2223 - A novel role of phospholipase A2 in mediating spinal cord secondary injury (2006)
Annals of Neurology, 59 (4), 606-619 - Degeneration of dopaminergic neurons induced by MPP+ in mouse mesencephalic dissociated culture (2006)
Journal of Jilin University Medicine Edition, 32 (5), 784-787 - Effect of aFGF with nuclear translocation sequence-knocked out on mitogenic activity to NIH3T3 cells and its mechanism (2006)
Journal of Jilin University Medicine Edition, 32 (5), 745-749 - Effect of human recombinant acidic fibroblast growth factor on growth and development in new-born rats (2006)
Journal of Jilin University Medicine Edition, 32 (4), 575-578 - Evidence for an oncogenic role of AHI-1 in Sezary syndrome, a leukemic variant of human cutaneous T-cell lymphomas (2006)
Leukemia, 20 (9), 1593-1601 - Expression and localization of p80 interleukin-1 receptor protein in the rat spinal cord (2006)
Journal of Molecular Neuroscience, 29 (1), 45-53 - Intermittent exposure of primitive quiescent chronic myeloid leukemia cells to granulocyte-colony stimulating factor in vitro promotes their elimination by imatinib mesylate (2006)
Clinical Cancer Research, 12 (2), 626-633 - Methods for isolating highly-enriched embryonic spinal cord neurons: A comparison between enzymatic and mechanical dissociations (2006)
Journal of Neuroscience Methods, 158 (1), 13-18 - Multiorgan engraftment and differentiation of human cord blood CD34 +Lin- cells in goats assessed by gene expression profiling (2006)
Proceedings of the National Academy of Sciences of the United States of America, 103 (20), 7801-7806 - Neuroprotective effects of citicoline on 6-hydroxydopamine-treated mesencephalic dopaminergic neurons in primary culture (2006)
Journal of Jilin University Medicine Edition, 32 (2) - Protective effect of panaxydol and panaxynol on sodium nitroprusside- induced apoptosis in cortical neurons (2006)
Chemico-Biological Interactions, 160 (3), 225-231 - BCR-ABL-transduced human cord blood cells produce abnormal populations in immunodeficient mice (2005)
Leukemia, 19 (3), 442-448 - Different subsets of primary chronic myeloid leukemia stem cells engraft immunodeficient mice and produce a model of the human disease (2005)
Leukemia, 19 (3), 435-441 - Protective effects of egg-milk with MT on mice damaged by irradiation (2005)
Journal of Jilin University Medicine Edition, 31 (4), 543-546 - Deregulated expression in Ph+ human leukemias of AHI-1, a gene activated by insertional mutagenesis in mouse models of leukemia (2004)
Blood, 103 (10), 3897-3904 - Growth autonomy and lineage switching in BCR-ABL-transduced human cord blood cells depend on different functional domains of BCR-ABL (2004)
Leukemia, 18 (5), 1006-1012 - Evidence for a positive role of SHIP in the BCR-ABL-mediated transformation of primitive murine hematopoietic cells and in human chronic myeloid leukemia (2003)
Blood, 102 (8), 2976-2984 - New models to investigate mechanisms of disease genesis from primitive BCR-ABL+ hematopoietic cells (2003)
Annals of the New York Academy of Sciences, 996, 1-9 - Stage-specific alterations in serum levels of G-CSF in chronic myeloid leukaemia [10] (2003)
Leukemia, 17 (7), 1430-1432 - Ahi-1, a novel gene encoding a modular protein with WD40-repeat and SH3 domains, is targeted by the Ahi-1 and Mis-2 provirus integrations (2002)
Journal of Virology, 76 (18), 9046-9059 - Elucidating critical mechanisms of deregulated stem cell turnover in the chronic phase of chronic myeloid leukemia (2002)
Leukemia, 16 (4), 549-558 - Modulation of p210 BCR-ABL activity in transduced primary human hematopoietic cells controls lineage programming (2002)
Blood, 99 (9), 3197-3204 - Primitive interleukin 3 null hematopoietic cells transduced with BCR-ABL show accelerated loss after culture of factor-independence in vitro and leukemogenic activity in vivo (2002)
Blood, 100 (10), 3731-3740 - Unfulfilled promise of endostatin in a gene therapy-xenotransplant model of human acute lymphocytic leukemia (2002)
Molecular Therapy, 5 (4), 352-359 - Primitive quiescent leukemic cells from patients with chronic myeloid leukemia spontaneously initiate factor-independent growth in vitro in association with up-regulation of expression of interleukin-3 (2001)
Blood, 97 (3), 720-728 - Autonomous multi-lineage differentiation in vitro of primitive CD34+ cells from patients with chronic myeloid leukemia (2000)
Leukemia, 14 (6), 1112-1121 - BCR-ABL transduction of multiple CD34* subsets of human cord blood cells leads to an excessive generation of erythroid progeny (2000)
Blood, 96 (11 PART I) - Reduced tyrosine phosphorylation of P210BCR ABL and lack of stats activation in BCR-ABL-transduced IL3' primitive hematopoietic cells (2000)
Blood, 96 (11 PART I) - Autocrine production and action of IL-3 and granulocyte colony-stimulating factor in chronic myeloid leukemia (1999)
Proceedings of the National Academy of Sciences of the United States of America, 96 (22), 12804-12809 - Isolation of a highly quiescent subpopulation of primitive leukemic cells in chronic myeloid leukemia (1999)
Blood, 94 (6), 2056-2064 - Study on inhibition and prevention of tumor and antioxidative effects of lithium carbonate in tumor bearing mice (1998)
Wei sheng yan jiu = Journal of hygiene research, 27 (2), 77-80 - The Myb and Ahi-1 genes are physically very closely linked on mouse Chromosome 10 (1994)
Mammalian Genome, 5 (3), 142-148 - Long-range mapping of Mis-2, a common provirus integration site identified in murine leukemia virus-induced thymomas and located 160 kilobase pairs downstream of myb (1993)
Journal of Virology, 67 (10), 5733-5739 - The Vin-1 gene, identified by provirus insertional mutagenesis, is the cyclin D2 (1993)
Oncogene, 8 (6), 1661-1666 - Chromosomal breakpoints within the first intron of the ABL gene are nonrandom in patients with chronic myelogenous leukemia (1990)
Blood, 76 (3), 597-601 - Studies of BCR and ABL gene rearrangements in chronic myelogenous leukemia patients by conventional and pulsed-field gel electrophoresis using gel inserts (1989)
Cancer Genetics and Cytogenetics, 42 (2), 287-294
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